Evidence map›Paper›PMID 33485325›Full record

ArticleBMC molecular and cell biology2021

The negative charge of the 343 site is essential for maintaining physiological functions of CXCR4.

Liqing Wang, Qiuhong Xiong, Ping Li, Guangxin Chen, Nayab Tariq, Changxin Wu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC molecular and cell biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Liqing WangInstitutes of Biomedical Sciences, Shanxi University, Taiyuan, 030006, China.
Qiuhong XiongInstitutes of Biomedical Sciences, Shanxi University, Taiyuan, 030006, China.
Ping LiInstitutes of Biomedical Sciences, Shanxi University, Taiyuan, 030006, China.
Guangxin ChenInstitutes of Biomedical Sciences, Shanxi University, Taiyuan, 030006, China.
Nayab TariqInstitutes of Biomedical Sciences, Shanxi University, Taiyuan, 030006, China.
Changxin WuInstitutes of Biomedical Sciences, Shanxi University, Taiyuan, 030006, China. cxw20@sxu.edu.cn.
Shanxi University · CN

Funding

Key Technologies Research and Development Program 2017YFD0500300Natural Science Foundation of Shanxi Province 201701D221152Natural Science Foundation of Shanxi Province 201801D221248Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi 2019L0007Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi 2019L0096
6 · The paper itself

Abstract

backgroundWarts, hypogammaglobulinemia, recurrent bacterial infections and myelokathexis (WHIM) syndrome is a primary immunodeficiency disease (PID) usually caused by autosomal dominant mutations in the chemokine receptor CXCR4 gene. To date, a total of nine different mutations including eight truncation mutations and one missense mutation (E343K, CXCR4

resultsWe completed a series of mutagenesis to generate different mutations at the 343 site of CXCR4 tail, and established a series of HeLa cell lines stably expressing CXCR4

conclusionTaken together, our results demonstrated that the negative charge at the 343 site of CXCR4 plays an essential role in regulating the down-stream signal transduction of CXCR4 for physiological events, and residue charge changes, no matter positive or neutral introduce aberrant activities and functions of CXCR4, thus consequently lead to WHIM syndrome.

Indexed as

Static ElectricityAmino Acid SequenceBase SequenceCell MovementHEK293 CellsHeLa CellsHumansInflammationMAP Kinase Signaling SystemMutationPhosphorylationPrimary Immunodeficiency DiseasesReceptors, CXCR4Structure-Activity RelationshipWartsCXCR4 protein, humanReceptors, CXCR4Abnormal activities of signal pathwayCXCR4MutationResidue charge changingWHIM

Identifiers

PMID33485325
PMCPMC7825245
OpenAlexW3125413539

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.