ArticleNature communications2021
Three-dimensional total-internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Brightness demixing for simultaneous multi-target imaging in 3D single-molecule localization microscopy.Nature methods · 2026Article
- Combined TIRF and 3D Super-Resolution Microscopy for Nanoscopic Characterization of Adhesion Molecules on Microvilli.Analytical chemistry · 2026Article
- Molecular-scale isotropic 3D super-resolution microscopy via interference localization.Nature methods · 2026Article
- 4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination.Nature methods · 2026Article
- Enhanced supercritical angle localization microscopy through point spread function modeling.Biomedical optics express · 2025Article
- Single-molecule localisation microscopy approaches reveal envelope glycoprotein clusters in single-enveloped viruses: a potential functional role?Biochemical Society transactions · 2025Review
- Chiral structural color from microdomes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Super-Resolution Goes Viral: T4 Virus Particles as Versatile 3D-Bio-NanoRulers.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Minimally Invasive DNA-Mediated Photostabilization for Extended Single-Molecule and Super-resolution Imaging.bioRxiv : the preprint server for biology · 2025Article
- Super-resolution imaging of the neuronal cytoskeleton.Npj imaging · 2024Review
- Triggered cagedSTORM microscopy.Biomedical optics express · 2024Article
- Combining pMINFLUX, graphene energy transfer and DNA-PAINT for nanometer precise 3D super-resolution microscopy.Light, science & applications · 2023Article
- Quantifying F-actin patches in single melanoma cells using total-internal reflection fluorescence microscopy.Scientific reports · 2022Article
- An alternative to MINFLUX that enables nanometer resolution in a confocal microscope.Light, science & applications · 2022Article
- Through the Eyes of Creators: Observing Artificial Molecular Motors.ACS nanoscience Au · 2022Review
- Expansion microscopy with ninefold swelling (NIFS) hydrogel permits cellular ultrastructure imaging on conventional microscope.Science advances · 2022Article
- Fluorescence nanoscopy at the sub-10 nm scale.Biophysical reviews · 2021Review
- Three-dimensional total-internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules.Nature communications · 2021Article
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Authors and funding
12 authors.
Funding
Abstract
Single-molecule localization microscopy enables far-field imaging with lateral resolution in the range of 10 to 20 nanometres, exploiting the fact that the centre position of a single-molecule's image can be determined with much higher accuracy than the size of that image itself. However, attaining the same level of resolution in the axial (third) dimension remains challenging. Here, we present Supercritical Illumination Microscopy Photometric z-Localization with Enhanced Resolution (SIMPLER), a photometric method to decode the axial position of single molecules in a total internal reflection fluorescence microscope. SIMPLER requires no hardware modification whatsoever to a conventional total internal reflection fluorescence microscope and complements any 2D single-molecule localization microscopy method to deliver 3D images with nearly isotropic nanometric resolution. Performance examples include SIMPLER-direct stochastic optical reconstruction microscopy images of the nuclear pore complex with sub-20 nm axial localization precision and visualization of microtubule cross-sections through SIMPLER-DNA points accumulation for imaging in nanoscale topography with sub-10 nm axial localization precision.
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