ArticleMicrobiome2021
Quantifying fluorescent glycan uptake to elucidate strain-level variability in foraging behaviors of rumen bacteria.
Article in Microbiome, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Alginate foraging is conserved in geographically and taxonomically distinct ruminant microbiomes.Nature communications · 2026Article
- Enhancing sheep rumen health with Przewalskia tangutica: boosting fermentation and microbial diversity in saline-alkali sown pastures.Animal microbiome · 2026Article
- Distribution of microbial carrageenan foraging pathways reveals a widespread latent trait within the ruminant intestinal microbiome.Nature communications · 2026Article
- Using phenotyping to visualize and identify selfish bacteria: a methods guide.Microbiology spectrum · 2025Article
- Activity-Based Tracking of Glycan Turnover in Microbiomes.Journal of the American Chemical Society · 2025Article
- Discovering microbiota functions via chemical probe incorporation for targeted sequencing.Current opinion in chemical biology · 2025Review
- Life history strategies complement niche partitioning to support the coexistence of closely related Gilliamella species in the bee gut.The ISME journal · 2025Article
- In vitro and ex vivo metabolism of chemically diverse fructans by bovine rumen Bifidobacterium and Lactobacillus species.Animal microbiome · 2024Article
- Effects of yeast culture supplementation on milk yield, rumen fermentation, metabolism, and bacterial composition in dairy goats.Frontiers in veterinary science · 2024Article
- Cell aggregation is associated with enzyme secretion strategies in marine polysaccharide-degrading bacteria.The ISME journal · 2023Article
- Characterizing the Effect of Amylase Inhibitors on Maltodextrin Metabolism by Gut Bacteria Using Fluorescent Glycan Labeling.ACS chemical biology · 2023Article
- Identifying glycan consumers in human gut microbiota samples using metabolic labeling coupled with fluorescence-activated cell sorting.Nature communications · 2023Article
- Visualization of Carbohydrate Uptake Using Fluorescent Polysaccharides.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Prebiotics and the Human Gut Microbiota: From Breakdown Mechanisms to the Impact on Metabolic Health.Nutrients · 2022Review
- Technical pipeline for screening microbial communities as a function of substrate specificity through fluorescent labelling.Communications biology · 2022Article
- Integrative interactomics applied to bovine fescue toxicosis.Scientific reports · 2022Article
- Fluorescence activated cell sorting and fermentation analysis to study rumen microbiome responses to administered live microbials and yeast cell wall derived prebiotics.Frontiers in microbiology · 2022Review
- Effect ofFrontiers in microbiology · 2022Article
- Approaches to Investigate Selective Dietary Polysaccharide Utilization by Human Gut Microbiota at a Functional Level.Frontiers in microbiology · 2021Review
- Polysaccharide-Bacteria Interactions From the Lens of Evolutionary Ecology.Frontiers in microbiology · 2021Article
Corrections and comments
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Authors and funding
15 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gut microbiomes, such as the microbial community that colonizes the rumen, have vast catabolic potential and play a vital role in host health and nutrition. By expanding our understanding of metabolic pathways in these ecosystems, we will garner foundational information for manipulating microbiome structure and function to influence host physiology. Currently, our knowledge of metabolic pathways relies heavily on inferences derived from metagenomics or culturing bacteria in vitro. However, novel approaches targeting specific cell physiologies can illuminate the functional potential encoded within microbial (meta)genomes to provide accurate assessments of metabolic abilities. Using fluorescently labeled polysaccharides, we visualized carbohydrate metabolism performed by single bacterial cells in a complex rumen sample, enabling a rapid assessment of their metabolic phenotype. Specifically, we identified bovine-adapted strains of Bacteroides thetaiotaomicron that metabolized yeast mannan in the rumen microbiome ex vivo and discerned the mechanistic differences between two distinct carbohydrate foraging behaviors, referred to as "medium grower" and "high grower." Using comparative whole-genome sequencing, RNA-seq, and carbohydrate-active enzyme fingerprinting, we could elucidate the strain-level variability in carbohydrate utilization systems of the two foraging behaviors to help predict individual strategies of nutrient acquisition. Here, we present a multi-faceted study using complimentary next-generation physiology and "omics" approaches to characterize microbial adaptation to a prebiotic in the rumen ecosystem. Video abstract.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.