ArticleParasites & vectors2021
Molecular characterization of Cryptosporidium isolates from humans in Ontario, Canada.
Article in Parasites & vectors, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 4 of them syntheses that pooled it.
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Who cites it
22 citing papers in PubMed, 4 syntheses or guidelines pooled it, 50 citations in OpenAlex.
- Cryptosporidium in Rabbits: A Global Systematic Review and Meta-Analysis of Prevalence, Species/Genotypes Distribution and Zoonotic Significance.Veterinary medicine and science · 2025Pooled it
- Foodborne and neglected parasitic zoonoses in Ethiopian red meat animals: insights from a systematic review and meta-analysis.Frontiers in veterinary science · 2025Pooled it
- Prevalence of human cryptosporidiosis in the Americas: systematic review and meta-analysis.Revista do Instituto de Medicina Tropical de Sao Paulo · 2022Pooled it
- Prevalence ofFrontiers in cellular and infection microbiology · 2021Pooled it
- Advancing genomics for waterborne pathogen surveillance in Australia.The Lancet regional health. Western Pacific · 2026Review
- Molecular characterization of Cryptosporidium spp. in goats (Capra hircus) from Zhejiang Province, China: evidence of zoonotic species and subtypes.BMC microbiology · 2026Article
- Article
- A review of recentCurrent research in parasitology & vector-borne diseases · 2025Review
- Multicopy subtelomeric genes underlie animal infectivity of divergent Cryptosporidium hominis subtypes.Nature communications · 2024Article
- Cryptosporidium parvum: an emerging occupational zoonosis in Finland.Acta veterinaria Scandinavica · 2023Article
- Prevalence, risk factors and molecular epidemiology of neonatal cryptosporidiosis in calves: The Argentine perspective.Current research in parasitology & vector-borne diseases · 2023Review
- Review
- Molecular Detection ofVeterinary sciences · 2022Article
- Fully resolved assembly of Cryptosporidium parvum.GigaScience · 2022Article
- Comparative evaluation of various diagnostic techniques for detection ofIranian journal of veterinary research · 2022Article
- Cross-Border Investigations on the Prevalence and Transmission Dynamics ofMicroorganisms · 2021Article
- An Update on ZoonoticAnimals : an open access journal from MDPI · 2021Review
- CryptoGenotyper: A new bioinformatics tool for rapidFood and waterborne parasitology · 2021Article
- High Diversity ofPathogens (Basel, Switzerland) · 2021Article
- Zoonotic parasites in farmed exotic animals in China: Implications to public health.International journal for parasitology. Parasites and wildlife · 2021Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCryptosporidiosis is a gastrointestinal disease with global distribution. It has been a reportable disease in Canada since 2000; however, routine molecular surveillance is not conducted. Therefore, sources of contamination are unknown. The aim of this project was to identify species and subtypes of Cryptosporidium in clinical cases from Ontario, the largest province in Canada, representing one third of the Canadian population, in order to understand transmission patterns.
methodsA total of 169 frozen, banked, unpreserved stool specimens that were microscopy positive for Cryptosporidium over the period 2008-2017 were characterized using molecular tools. A subset of the 169 specimens were replicate samples from individual cases. DNA was extracted directly from the stool and nested PCR followed by Sanger sequencing was conducted targeting the small subunit ribosomal RNA (SSU) and glycoprotein 60 (gp60) genes.
resultsMolecular typing data and limited demographic data were obtained for 129 cases of cryptosporidiosis. Of these cases, 91 (70.5 %) were due to Cryptosporidium parvum and 24 (18.6%) were due to Cryptosporidium hominis. Mixed infections of C. parvum and C. hominis occurred in four (3.1%) cases. Five other species observed were Cryptosporidium ubiquitum (n = 5), Cryptosporidium felis (n = 2), Cryptosporidium meleagridis (n = 1), Cryptosporidium cuniculus (n = 1) and Cryptosporidium muris (n = 1). Subtyping the gp60 gene revealed 5 allelic families and 17 subtypes of C. hominis and 3 allelic families and 17 subtypes of C. parvum. The most frequent subtype of C. hominis was IbA10G2 (22.3%) and of C. parvum was IIaA15G2R1 (62.4%).
conclusionsThe majority of isolates in this study were C. parvum, supporting the notion that zoonotic transmission is the main route of cryptosporidiosis transmission in Ontario. Nonetheless, the observation of C. hominis in about a quarter of cases suggests that anthroponotic transmission is also an important contributor to cryptosporidiosis pathogenesis in Ontario.
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