Evidence map›Paper›PMID 33482722›Full record

ArticleBMC microbiology2021

Revealing biophysical properties of KfrA-type proteins as a novel class of cytoskeletal, coiled-coil plasmid-encoded proteins.

M Adamczyk, E Lewicka, R Szatkowska, H Nieznanska, J Ludwiczak, M Jasiński, S Dunin-Horkawicz, E Sitkiewicz, B Swiderska, G Goch and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. A Plasmid CarryingMicroorganisms · 2022
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 1 country.

M AdamczykWarsaw University of Technology, Faculty of Chemistry, Chair of Drug and Cosmetics Biotechnology, Noakowskiego 3, 00-664, Warsaw, Poland. madamczyk@ch.pw.edu.pl.ORCID 0000-0003-3979-0364
E LewickaDepartment of Microbial Biochemistry, Institute of Biochemistry and Biophysics PAS, Pawinskiego 5a, 02-106, Warsaw, Poland.
R SzatkowskaWarsaw University of Technology, Faculty of Chemistry, Chair of Drug and Cosmetics Biotechnology, Noakowskiego 3, 00-664, Warsaw, Poland.
H NieznanskaNencki Institute of Experimental Biology PAS, Laboratory of Electron Microscopy, Pasteura 3, 02-093, Warsaw, Poland.
J LudwiczakUniversity of Warsaw, Centre of New Technologies, Laboratory of Structural Bioinformatics, 02-097, Warsaw, Poland.
M JasińskiUniversity of Warsaw, Centre of New Technologies, Laboratory of Structural Bioinformatics, 02-097, Warsaw, Poland.
S Dunin-HorkawiczUniversity of Warsaw, Centre of New Technologies, Laboratory of Structural Bioinformatics, 02-097, Warsaw, Poland.
E SitkiewiczMass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106, Warsaw, Poland.
B SwiderskaMass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106, Warsaw, Poland.
G GochDepartment of Microbial Biochemistry, Institute of Biochemistry and Biophysics PAS, Pawinskiego 5a, 02-106, Warsaw, Poland.
G Jagura-BurdzyDepartment of Microbial Biochemistry, Institute of Biochemistry and Biophysics PAS, Pawinskiego 5a, 02-106, Warsaw, Poland.
Institute of Biochemistry and Biophysics, Polish Academy of Sciences · PLUniversity of Warsaw · PLWarsaw University of Technology · PLInstytut Biologii Doświadczalnej im. Marcelego Nenckiego · PLInstytut Biologii Doświadczalnej im. Marcelego Nenckiego · PL

Funding

Interdisciplinary Centre for Mathematical and Computational Modeling (ICM) University of Warsaw GA71-24 to SDHNarodowe Centrum Nauki 2015/17/B/NZ2/01160Narodowe Centrum Nauki 2019/32/T/NZ1/00323
6 · The paper itself

Abstract

backgroundDNA binding KfrA-type proteins of broad-host-range bacterial plasmids belonging to IncP-1 and IncU incompatibility groups are characterized by globular N-terminal head domains and long alpha-helical coiled-coil tails. They have been shown to act as transcriptional auto-regulators.

resultsThis study was focused on two members of the growing family of KfrA-type proteins encoded by the broad-host-range plasmids, R751 of IncP-1β and RA3 of IncU groups. Comparative in vitro and in silico studies on KfrA

conclusionKfrA

Indexed as

Bacterial ProteinsBinding SitesComputer SimulationConjugation, GeneticCytoskeletal ProteinsDNA-Binding ProteinsEscherichia coliPlasmidsTranscription, GeneticBacterial ProteinsCytoskeletal ProteinsDNA-Binding ProteinsBroad-host-range plasmidsBrownian motionCoiled-coil proteinsDNA-protein interactionSelf-assemblyStability functions

Identifiers

PMID33482722
PMCPMC7821693
OpenAlexW3123436849

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.