ArticleBMC genomics2021
BSR-Seq analysis provides insights into the cold stress response of Actinidia arguta F1 populations.
Article in BMC genomics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- AaSIZ1-Mediated SUMOylation of AaMYB31 Positively Regulates Freezing Tolerance in Actinidia arguta.Plant biotechnology journal · 2026Article
- Identification of QTLs and Key Genes Enhancing Lodging Resistance in Soybean Through Chemical and Physical Trait Analysis.Plants (Basel, Switzerland) · 2024Article
- Identification of Cold Tolerance Transcriptional Regulatory Genes in Seedlings ofInternational journal of molecular sciences · 2024Article
- Haplotype-resolved genome assembly provides insights into evolutionary history of the Actinidia arguta tetraploid.Molecular horticulture · 2024Article
- Allelically and Differentially Expressed Genes After Infection of Edwardsiella ictaluri in Channel Catfish as Determined by Bulk Segregant RNA-Seq.Marine biotechnology (New York, N.Y.) · 2022Article
- Mechanism of cotton resistance to abiotic stress, and recent research advances in the osmoregulation related genes.Frontiers in plant science · 2022Review
- Harnessing the potential of bulk segregant analysis sequencing and its related approaches in crop breeding.Frontiers in genetics · 2022Review
- Genomics and Pathways Involved in Maize Resistance toFrontiers in plant science · 2022Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundFreezing injury, which is an important abiotic stress in horticultural crops, influences the growth and development and the production area of kiwifruit (Actinidia Lind1). Among Actinidia species, Actinidia arguta has excellent cold resistance, but knowledge relevant to molecular mechanisms is still limited. Understanding the mechanism underlying cold resistance in kiwifruit is important for breeding cold resistance.
resultsIn our study, a population resulting from the cross of A. arguta 'Ruby-3' × 'Kuilv' male was generated for kiwifruit hardiness study, and 20 cold-tolerant and 20 cold-sensitive populations were selected from 492 populations according to their LT50. Then, we performed bulked segregant RNA-seq combined with single-molecule real-time sequencing to identify differentially expressed genes that provide cold hardiness. We found that the content of soluble sucrose and the activity of β-amylase were higher in the cold-tolerant population than in the cold-sensitive population. Upon - 30 °C low-temperature treatment, 126 differentially expressed genes were identify; the expression of 59 genes was up-regulated and that of 67 genes was down-regulated between the tolerant and sensitive pools, respectively. KEGG pathway analysis showed that the DEGs were primarily related to starch and sucrose metabolism, amino sugar and nucleotide sugar metabolism. Ten major key enzyme-encoding genes and two regulatory genes were up-regulated in the tolerant pool, and regulatory genes of the CBF pathway were found to be differentially expressed. In particular, a 14-3-3 gene was down-regulated and an EBF gene was up-regulated. To validate the BSR-Seq results, 24 DEGs were assessed via qRT-PCR, and the results were consistent with those obtained by BSR-Seq.
conclusionOur research provides valuable insights into the mechanism related to cold resistance in Actinidia and identified potential genes that are important for cold resistance in kiwifruit.
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