ArticleStem cell reports2021
Gastruloid Development Competence Discriminates Different States of Pluripotency.
Article in Stem cell reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed.
- A quantitative pipeline for whole-mount deep imaging and analysis of multi-layered organoids across scales.eLife · 2026Article
- Investigation of embryogenesis in a dish: the development and application of gastruloid models from pluripotent stem cells.Cell & bioscience · 2026Review
- R-loops shape the H2A.Z landscape and promote balanced lineage allocation during differentiation.Genome research · 2026Article
- Non-cell-autonomous control of mouse gastruloid development by the ultra-conserved lncRNA T-UCstem1.The EMBO journal · 2025Article
- Gastruloid patterning reflects division of labor among biased stem cell clones.bioRxiv : the preprint server for biology · 2025Article
- The Modulation of Cell Plasticity by Budesonide: Beyond the Metabolic and Anti-Inflammatory Actions of Glucocorticoids.Pharmaceutics · 2025Review
- Stem cell culture conditions affect in vitro differentiation potential and mouse gastruloid formation.PloS one · 2025Article
- In vitro modelling of anterior primitive streak patterning with human pluripotent stem cells identifies the path to notochord progenitors.Development (Cambridge, England) · 2024Article
- Three-dimensional stem cell models of mammalian gastrulation.BioEssays : news and reviews in molecular, cellular and developmental biology · 2024Review
- Early autonomous patterning of the anteroposterior axis in gastruloids.Development (Cambridge, England) · 2024Article
- Article
- BPA Exposure Affects Mouse Gastruloids Axial Elongation by Perturbing the Wnt/β-Catenin Pathway.International journal of molecular sciences · 2024Article
- The shapes of elongating gastruloids are consistent with convergent extension driven by a combination of active cell crawling and differential adhesion.PLoS computational biology · 2024Article
- Using Embryo Models to Understand the Development and Progression of Embryonic Lineages: A Focus on Primordial Germ Cell Development.Cells, tissues, organs · 2024Review
- Gastruloids - a minimalistic model to study complex developmental metabolism.Emerging topics in life sciences · 2023Review
- The many dimensions of germline competence.Current opinion in cell biology · 2023Review
- Signalling pathway crosstalk stimulated by L-proline drives mouse embryonic stem cells to primitive-ectoderm-like cells.Development (Cambridge, England) · 2023Article
- Gastruloid-derived primordial germ cell-like cells develop dynamically within integrated tissues.Development (Cambridge, England) · 2023Article
- Budesonide Analogues Preserve Stem Cell Pluripotency and Delay 3D Gastruloid Development.Pharmaceutics · 2023Article
- Multimodal characterization of murine gastruloid development.Cell stem cell · 2023Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Floating spheroidal aggregates of mouse embryonic stem cells can develop into polarized/elongated organoids, namely gastruloids. We set up a high-performing assay to measure gastruloid formation efficiency (GFE), and found that GFE decreases as pluripotency progresses from naive (GFE ≥ 95%) to primed (GFE = 0) state. Specifically, we show that primed EpiSCs fail to generate proper cell aggregates, while early-primed EpiLCs aggregate but eventually fail to develop into elongated gastruloids. Moreover, we characterized proline-induced cells (PiCs), a LIF-dependent reversible early-primed state of pluripotency, and show that PiCs are able to generate gastruloids (GFE ∼ 50%) and are also competent to differentiate into primordial germ cell-like cells. Thus, we propose the GFE assay as a valuable functional tool to discriminate different states of the pluripotency continuum.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.