Evidence map›Paper›PMID 33481244›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Fluorogenic XY-69 in Lipid Vesicles for Measuring Activity of Phospholipase C Isozymes.

Adam J Carr, Edhriz Siraliev-Perez, Weigang Huang, John Sondek, Qisheng Zhang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
3.1field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 8 citations in OpenAlex.

  1. Anionic lipids regulate PLCbioRxiv : the preprint server for biology · 2026
    Article
  2. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Adam J CarrDivision of Chemical Biology and Medicinal Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Edhriz Siraliev-PerezDepartment of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Weigang HuangDivision of Chemical Biology and Medicinal Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
John SondekDepartment of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Qisheng ZhangDivision of Chemical Biology and Medicinal Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. qszhang@unc.edu.
University of North Carolina at Chapel Hill · US

Funding

Regulation of Phospholipase CR01GM057391 · NIGMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI SONDEK, JOHN E · 1998 to 2021
$6.9M
Single-Cell Measurement of Lipid Signaling in Colorectal CancerR01CA177993 · NCI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI ALLBRITTON, NANCY L. · 2014 to 2018
$3.0M
NCI NIH HHS R01 CA177993NIGMS NIH HHS R01 GM057391
6 · The paper itself

Abstract

Mammalian phospholipase C (PLC) isozymes are major signaling nodes that regulate a wide range of cellular processes. Dysregulation of PLC activity has been associated with a growing list of human diseases such as cancer and Alzheimer's disease. However, methods to directly and continuously monitor PLC activity at membranes with high sensitivity and throughput are still lacking. We have developed XY-69, a fluorogenic PIP

Indexed as

Enzyme AssaysFluorescein-5-isothiocyanateHydrolysisIsoenzymesLipid MetabolismLipidsPhosphatidylinositol 4,5-DiphosphatePhospholipase C gammaProtein BindingType C PhospholipasesFluorescein-5-isothiocyanateIsoenzymesLipidsPhosphatidylinositol 4,5-DiphosphatePhospholipase C gammaType C PhospholipasesEnzymatic assayFluorogenic reporterHigh-throughput screenLipid vesiclesphosphatidylinositol 4,5-bisphosphatePhospholipase C

Identifiers

PMID33481244
PMCPMC8094607
OpenAlexW3124785001

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.