Evidence map›Paper›PMID 33481228›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Simultaneous Detection of Phosphoinositide Lipids by Radioactive Metabolic Labeling.

Noah Steinfeld, Sai Srinivas Panapakkam Giridharan, Emily J Kauffman, Lois S Weisman

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Quantitative comparison of PI(3,5)PMolecular biology of the cell · 2026
    Article
  2. Article
  3. Article
  4. Quantitative comparison of PI(3,5)PbioRxiv : the preprint server for biology · 2025
    Article
  5. Review
  6. Article
  7. Article
  8. Regulation of yeast polarized exocytosis by phosphoinositide lipids.Cellular and molecular life sciences : CMLS · 2024
    Review
  9. Roles of PIKfyve in multiple cellular pathways.Current opinion in cell biology · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Noah Steinfeld *Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.
Sai Srinivas Panapakkam Giridharan *Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.
Emily J KauffmanLife Sciences Institute, University of Michigan, Ann Arbor, MI, USA.
Lois S WeismanLife Sciences Institute, University of Michigan, Ann Arbor, MI, USA. lweisman@umich.edu.

Funding

CELLULAR AND MOLECULAR BIOLOGY AT MICHIGANT32GM007315 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI PUTHENVEEDU, MANOJKUMAR A · 1985 to 2021
$12.8M
Roles and regulation of PI(3,5)P2 and PI5P in neuronsR01NS064015 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI WEISMAN, LOIS S · 2009 to 2018
$3.8M
Phosphoinositide signaling: novel potential targets for Huntington diseaseR01NS099340 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI WEISMAN, LOIS S · 2017 to 2021
$2.7M
NIGMS NIH HHS T32 GM007315NINDS NIH HHS R01 NS064015NINDS NIH HHS R01 NS099340
6 · The paper itself

Abstract

Phosphoinositide (PPI) lipids are a crucial class of low-abundance signaling molecules that regulate many processes within cells. Methods that enable simultaneous detection of all PPI lipid species provide a wholistic snapshot of the PPI profile of cells, which is critical for probing PPI biology. Here we describe a method for the simultaneous measurement of cellular PPI levels by metabolically labeling yeast or mammalian cells with myo-

Indexed as

AnimalsBiochemical PhenomenaHumansInositolIsotope LabelingPhosphatidylinositol 3-KinasesPhosphatidylinositol PhosphatesPhosphatidylinositolsRadioisotopesSaccharomyces cerevisiaeSignal TransductionInositolPhosphatidylinositol 3-KinasesPhosphatidylinositol PhosphatesPhosphatidylinositolsRadioisotopesPhosphatidylinositolPhosphoinositide lipidsPI3,4,5P3PI3,4P2PI3,5P2PI3PPI4,5P2PI4PPI5PPtdInsRadioactive metabolic labeling

Identifiers

PMID33481228
PMCPMC8059495

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.