Evidence map›Paper›PMID 33481075›Full record

ArticleBioprocess and biosystems engineering2021

Construction of recombinant Escherichia coli expressing xylitol-4-dehydrogenase and optimization for enhanced L-xylulose biotransformation from xylitol.

Mesfin Angaw Tesfay, Xin Wen, Yujie Liu, Huibin Lin, Linxu Chen, Jianqiang Lin, Jianqun Lin

Abstract read
PubMed Publisher
In one paragraph

Article in Bioprocess and biosystems engineering, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Characterization of a xylitol dehydrogenase fromFrontiers in bioengineering and biotechnology · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Mesfin Angaw TesfayState Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
Xin WenState Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
Yujie LiuState Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
Huibin LinShandong Academy of Chinese Medicine, Jinan, 250014, China.
Linxu ChenState Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
Jianqiang LinState Key Laboratory of Microbial Technology, Shandong University, Qingdao, China. jianqianglin@sdu.edu.cn.
Jianqun LinState Key Laboratory of Microbial Technology, Shandong University, Qingdao, China. jianqunlin@sdu.edu.cn.
Shandong University · CNShandong Academy of Chinese Medicine · CNState Key Laboratory of Microbial Technology

Funding

Key R & D Plan of Shandong Province in 2019, China 2019GSF107015National Key Research and Development project of China 2017YFC1701502,2017YFC1701504Shandong Province Science and Technology Development Project, China 2015GSF121016
6 · The paper itself

Abstract

L-Xylulose is a rare ketopentose which inhibits α-glucosidase and is an indicator of hepatitis or liver cirrhosis. This pentose is also a precursor of other rare sugars such as L-xylose, L-ribose or L-lyxose. Recombinant E. coli expressing xylitol-4-dehydrogenase gene of Pantoea ananatis was constructed. A cost-effective culture media were used for L-xylulose production using the recombinant E. coli strain constructed. Response surface methodology was used to optimize these media components for L-xylulose production. A high conversion rate of 96.5% was achieved under an optimized pH and temperature using 20 g/L xylitol, which is the highest among the reports. The recombinant E. coli cells expressing the xdh gene were immobilized in calcium alginate to improve recycling of cells. Effective immobilization was achieved with 2% (w/v) sodium alginate and 3% (w/v) calcium chloride. The immobilized E. coli cells retained good stability and enzyme activity for 9 batches with conversion between 53 and 92% which would be beneficial for economical production of L-xylulose.

Indexed as

Bacterial ProteinsD-Xylulose ReductaseEscherichia coliMicroorganisms, Genetically-ModifiedPantoeaXylitolXyluloseBacterial ProteinsD-Xylulose ReductaseXylitolXyluloseCell immobilizationL-xyluloseMedia optimizationRecombinant Escherichia coli

Identifiers

PMID33481075
OpenAlexW3122904237

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.