ArticleSoft matter2021
An expanding bacterial colony forms a depletion zone with growing droplets.
Article in Soft matter, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- A Compact Schlieren Optics Device for Imaging Biological Samples.Bio-protocol · 2026Article
- Toothbrush-Driven Handheld Droplet Generator for Digital LAMP and Rapid CFU Assays.Biosensors · 2026Article
- Active interface bulging inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Forced Wetting Properties of Bacteria-Laden Droplets Experiencing Initial Evaporation.Langmuir : the ACS journal of surfaces and colloids · 2023Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Many species of bacteria have developed effective means to spread on solid surfaces. This study focuses on the expansion of Pseudomonas aeruginosa on an agar gel surface under conditions of minimal evaporation. We report the occurrence and spread of a depletion zone within an expanded colony, where the bacteria laden film becomes thinner. The depletion zone is colocalized with a higher concentration of rhamnolipids, the biosurfactants that are produced by the bacteria and accumulate in the older region of the colony. With continued growth in population, dense bacterial droplets occur and coalesce in the depletion zone, displaying remarkable fluid dynamic behavior. Whereas expansion of a central depletion zone requires activities of live bacteria, new zones can be seeded elsewhere by adding rhamnolipids. These depletion zones due to the added surfactants expand quickly, even on plates covered by bacteria that have been killed by ultraviolet light. We explain the observed properties based on considerations of bacterial growth and secretion, osmotic swelling, fluid volume expansion, interfacial fluid dynamics involving Marangoni and capillary flows, and cell-cell cohesion.
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Registered trials
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