Evidence map›Paper›PMID 33479594›Full record

Article3 Biotech2021

3-Methyl-2-benzothiazolinone hydrazone and 3-dimethylamino benzoic acid as substrates for the development of polyphenoloxidase and phenoloxidase activity by zymograms.

Y García-Esquivel, Y Mercado-Flores, M A Anducho-Reyes, J Álvarez-Cervantes, E Aguirre-von Wobeser, A I Marina-Ramírez, A Téllez-Jurado

Open access · greenAbstract read
In one paragraph

Article in 3 Biotech, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 3 countries.

Y García-EsquivelLaboratorio de AgroBiotecnología, Universidad Politécnica de Pachuca, Carretera Pachuca-Cd. Sahagún, km 20, ExHacienda de Santa Bárbara, C.P. 43830 Zempoala, Hidalgo México.
Y Mercado-FloresLaboratorio de AgroBiotecnología, Universidad Politécnica de Pachuca, Carretera Pachuca-Cd. Sahagún, km 20, ExHacienda de Santa Bárbara, C.P. 43830 Zempoala, Hidalgo México.
M A Anducho-ReyesLaboratorio de AgroBiotecnología, Universidad Politécnica de Pachuca, Carretera Pachuca-Cd. Sahagún, km 20, ExHacienda de Santa Bárbara, C.P. 43830 Zempoala, Hidalgo México.
J Álvarez-CervantesLaboratorio de AgroBiotecnología, Universidad Politécnica de Pachuca, Carretera Pachuca-Cd. Sahagún, km 20, ExHacienda de Santa Bárbara, C.P. 43830 Zempoala, Hidalgo México.
E Aguirre-von WobeserCátedras CONACyT, Centro de Investigación en Alimentos y Desarrollo A.C., Centro de Investigación en Agrobiotecnología Alimentaria, Cd. del Conocimiento, Boulevard Circuito La Concepción, C.P. 42162 San Agustín Tlaxiaca, Hidalgo México.
A I Marina-RamírezProteómica, Centro de Biología Molecular Severo Ochoa, Nicolás Cabrera No. 1, C.P. 28049 Madrid, Spain.
A Téllez-JuradoLaboratorio de AgroBiotecnología, Universidad Politécnica de Pachuca, Carretera Pachuca-Cd. Sahagún, km 20, ExHacienda de Santa Bárbara, C.P. 43830 Zempoala, Hidalgo México.ORCID 0000-0002-5491-3679
Universidad Politécnica de Pachuca · MXCentro de Biología Molecular Severo Ochoa · ESCentro de Investigación en Alimentación y Desarrollo · MX

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the present study, a sequential staining process of polyphenoloxidase and phenoloxidase enzymes was designed by the zymography technique. As a first step, electrophoresis was carried out under native conditions, and later, first staining was carried out with a revealing solution of 3-methyl-2-benzothiazoline hydrazone (MBTH)-3-dimethylamino benzoic acid (DMAB) that allowed the visualization of polyphenoloxidase enzymes, and later and using the same gel, we proceeded to the differential staining of phenoloxidase, adding a solution of H

Indexed as

DMABIsoenzymesLaccaseMBTHPhenoloxidaseZymography

Identifiers

PMID33479594
PMCPMC7794263
OpenAlexW3120165936

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.