ArticleScientific reports2021
Analysis of ovarian transcriptomes reveals thousands of novel genes in the insect vector Rhodnius prolixus.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- Loss of vitellogenin receptor function results in yolk depletion, virome expansion, and reduced bacterial load within the oocytes of Rhodnius prolixus.BMC biology · 2026Article
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- Refining the annotation ofCurrent research in parasitology & vector-borne diseases · 2025Article
- ACurrent research in insect science · 2024Article
- Embryonic piRNAs target horizontally transferred vertebrate transposons in assassin bugs.Frontiers in cell and developmental biology · 2024Article
- Knockdown of Sec16 causes early lethality and defective deposition of the protein Rp30 in the eggshell of the vectorFrontiers in cell and developmental biology · 2024Article
- A pro-BMP function exerted byOpen biology · 2023Article
- Silencing of the 20S proteasomal subunit-α6 triggers full oogenesis arrest and increased mRNA levels of the selective autophagy adaptor protein p62/SQSTM1 in the ovary of the vector Rhodnius prolixus.PLoS neglected tropical diseases · 2023Article
- Lactobacillus for ribosome peptide editing cancer.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2023Review
- Global characterization of gene expression in the brain of starved immature Rhodnius prolixus.PloS one · 2023Article
- Functional characterization of maternally accumulated hydrolases in the mature oocytes of the vectorFrontiers in physiology · 2023Article
- Dynamics of maternal gene expression in Rhodnius prolixus.Scientific reports · 2022Article
- Annotations of novel antennae-expressed genes in male Glossina morsitans morsitans tsetse flies.PloS one · 2022Article
- Salivary and Intestinal Transcriptomes Reveal Differential Gene Expression in Starving, Fed andFrontiers in cellular and infection microbiology · 2021Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Rhodnius prolixus is a Triatominae insect species and a primary vector of Chagas disease. The genome of R. prolixus has been recently sequenced and partially assembled, but few transcriptome analyses have been performed to date. In this study, we describe the stage-specific transcriptomes obtained from previtellogenic stages of oogenesis and from mature eggs. By analyzing ~ 228 million paired-end RNA-Seq reads, we significantly improved the current genome annotations for 9206 genes. We provide extended 5' and 3' UTRs, complete Open Reading Frames, and alternative transcript variants. Strikingly, using a combination of genome-guided and de novo transcriptome assembly we found more than two thousand novel genes, thus increasing the number of genes in R. prolixus from 15,738 to 17,864. We used the improved transcriptome to investigate stage-specific gene expression profiles during R. prolixus oogenesis. Our data reveal that 11,127 genes are expressed in the early previtellogenic stage of oogenesis and their transcripts are deposited in the developing egg including key factors regulating germline development, genome integrity, and the maternal-zygotic transition. In addition, GO term analyses show that transcripts encoding components of the steroid hormone receptor pathway, cytoskeleton, and intracellular signaling are abundant in the mature eggs, where they likely control early embryonic development upon fertilization. Our results significantly improve the R. prolixus genome and transcriptome and provide novel insight into oogenesis and early embryogenesis in this medically relevant insect.
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