ArticleScientific reports2021
Fully integrated rapid microfluidic device translated from conventional 96-well ELISA kit.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.
- Unveiling the state of the art: a systematic review and meta-analysis of paper-based microfluidic devices.Frontiers in bioengineering and biotechnology · 2024Pooled it
- Development of 3D printed capillaric circuit interfacing with silver nanoparticle embedded membrane for simultaneous analysis of duplex samples via fluorescence sandwich immunoassays.Analytical and bioanalytical chemistry · 2026Article
- Research Advances and Future Perspectives of Point-of-Care Detection Technologies and Biosensors for Mosquito-Borne Viruses.Biosensors · 2026Review
- AI-driven lateral flow immunoassay for point-of-care detection of cardiac biomarkers in acute myocardial infarction.Mikrochimica acta · 2026Article
- Recent Advances in Microfluidic Chip Technology for Laboratory Medicine: Innovations and Artificial Intelligence Integration.Biosensors · 2026Review
- A One-Step-Packaged CMOS/Microfluidics Wireless Bio-Analyzer for Point-of-Care Diagnostics.IEEE journal of solid-state circuits · 2025Article
- Development of microfluidic ELISA for measuring humoral responses to clostridial antigens in vaccinated cattle.Journal of immunological methods · 2025Article
- Review
- Allele-specific electrical genotyping for diagnosis of transthyretin amyloidosis.Communications engineering · 2025Article
- Automated Electrical Detection of Proteins for Oral Squamous Cell Carcinoma in an Integrated Microfluidic Chip Using Multi-Frequency Impedance Cytometry and Machine Learning.Sensors (Basel, Switzerland) · 2025Article
- Revolutionary Point-of-Care Wearable Diagnostics for Early Disease Detection and Biomarker Discovery through Intelligent Technologies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Rapid deep learning-assisted predictive diagnostics for point-of-care testing.Nature communications · 2024Article
- Development of a C-reactive protein quantification method based on flow rate measurement of an ink solution pushed out by oxygen gas generated by catalase reaction.Mikrochimica acta · 2023Article
- An Internet-of-Disease System for COVID-19 Testing Using Saliva by an AI-Controlled Microfluidic ELISA Device.Advanced materials technologies · 2022Article
- Rapid Detection of Direct Compound Toxicity and Trailing Detection of Indirect Cell Metabolite Toxicity in a 96-Well Fluidic Culture Device for Cell-Based Screening Environments: Tactics in Six Sigma Quality Control Charts.Applied sciences (Basel, Switzerland) · 2022Article
- Microfluidic Point-of-Care (POC) Devices in Early Diagnosis: A Review of Opportunities and Challenges.Sensors (Basel, Switzerland) · 2022Review
- Pushing the detection limits: strategies towards highly sensitive optical-based protein detection.Analytical and bioanalytical chemistry · 2021Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
In this work, a fully integrated active microfluidic device transforming a conventional 96-well kit into point-of-care testing (POCT) device was implemented to improve the performance of traditional enzyme-linked immunosorbent assay (ELISA). ELISA test by the conventional method often requires the collection of 96 samples for its operation as well as longer incubation time from hours to overnight, whereas our proposed device conducts ELISA immediately individualizing a 96-well for individual patients. To do that, a programmable and disposable on-chip pump and valve were integrated on the device for precise control and actuation of microfluidic reagents, which regulated a reaction time and reagent volume to support the optimized protocols of ELISA. Due to the on-chip pump and valve, ELISA could be executed with reduced consumption of reagents and shortening the assay time, which are crucial for conventional ELISA using 96-well microplate. To demonstrate highly sensitive detection and easy-to-use operation, this unconventional device was successfully applied for the quantification of cardiac troponin I (cTnI) of 4.88 pg/mL using a minimum sample volume of 30 µL with a shorter assay time of 15 min for each ELISA step. The limit of detection (LOD) thus obtained was significantly improved than the conventional 96-well platform.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.