Evidence map›Paper›PMID 33479281›Full record

ArticleScientific reports2021

Isolation of Borrelia miyamotoi and other Borreliae using a modified BSK medium.

Adam J Replogle, Christopher Sexton, John Young, Luke C Kingry, Martin E Schriefer, Marc Dolan, Tammi L Johnson, Neeta P Connally, Kerry A Padgett, Jeannine M Petersen

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
5.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 33 citations in OpenAlex.

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  12. Pathogens (Basel, Switzerland) · 2023
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  13. Molecular detection and identification of relapsing feverEmerging microbes & infections · 2022
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  15. iScience · 2022
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  19. Transovarial Transmission ofMicroorganisms · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Adam J ReplogleDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, 80521, USA.
Christopher SextonDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, 80521, USA.
John YoungDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, 80521, USA.
Luke C KingryDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, 80521, USA.
Martin E SchrieferDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, 80521, USA.
Marc DolanDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, 80521, USA.
Tammi L JohnsonDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, 80521, USA.
Neeta P ConnallyDepartment of Biological and Environmental Sciences, Western Connecticut State University, Danbury, CT, 06810, USA.
Kerry A PadgettCalifornia Department of Public Health, Infectious Diseases Branch/Vector-Borne Disease Section, Marina Bay Parkway, Richmond, CA, 94804, USA.
Jeannine M PetersenDivision of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, 80521, USA. nzp0@cdc.gov.
Centers for Disease Control and Prevention · USCalifornia Department of Public Health · USWestern Connecticut State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Borrelia spirochetes are the causative agents of Lyme borreliosis (LB) and relapsing fever (RF). Despite the steady rise in infections and the identification of new species causing human illness over the last decade, isolation of borreliae in culture has become increasingly rare. A modified Barbour-Stoenner-Kelly (BSK) media formulation, BSK-R, was developed for isolation of the emerging RF pathogen, Borrelia miyamotoi. BSK-R is a diluted BSK-II derivative supplemented with Lebovitz's L-15, mouse and fetal calf serum. Decreasing the concentration of CMRL 1066 and other components was essential for growth of North American B. miyamotoi. Sixteen B. miyamotoi isolates, originating from Ixodes scapularis ticks, rodent and human blood collected in the eastern and upper midwestern United States, were isolated and propagated to densities > 10

Indexed as

AnimalsBorreliaBorrelia burgdorferiCulture MediaHumansIxodesLyme DiseaseMiceRelapsing FeverSpirochaetalesCulture Media

Identifiers

PMID33479281
PMCPMC7820315
OpenAlexW3124929539

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.