ArticleNature communications2021
Sarcoma classification by DNA methylation profiling.
Registry-linked trialOpen access · goldAbstract read
In one paragraph
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06140992 (PaCIFiC-CUP：Pan-Cancer Integrated Fingerprinting Classifier for Identifying the Origin of Cancer of Unknown Primary), which is not on this map. Cited by 293 papers, 2 of them syntheses that pooled it.
0numbers the graph read from it
0cells of the map it votes in
293citing papers in PubMed, 2 pooled it
60.3field-weighted citation impact, top 1% of its field
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
PaCIFiC-CUP：Pan-Cancer Integrated Fingerprinting Classifier for Identifying the Origin of Cancer of Unknown Primary: A Multi-Center Prospective Cohort Study
Who cites it
293 citing papers in PubMed, 2 syntheses or guidelines pooled it, 521 citations in OpenAlex.
- Artificial-Intelligence-Based Radiologic, Histopathologic, and Molecular Models for the Diagnosis and Classification of Malignant Salivary Gland Tumors: A Systematic Review and Functional Meta-Synthesis.Medical sciences (Basel, Switzerland) · 2026Pooled it
- DNA Methylation-Based Classification for Central Nervous System Tumours: A Health Technology Assessment.Ontario health technology assessment series · 2025Pooled it
- The ITCC-P4 PDX Platform Enables Preclinical Testing of Pediatric Cancers.Cancer research · 2026Article
- Primary CNS sarcoma, DICER1-mutant with rhabdomyoblastic differentiation arising within the thoracic spine: illustrative case.Journal of neurosurgery. Case lessons · 2026Article
- CpG hypermethylation and WNT/AP-1 cooperativity define the epigenetic landscape and a clinical subgroup of high-risk pediatric adrenocortical carcinoma.Nature communications · 2026Article
- Spindle Cell Tumors with aInternational journal of molecular sciences · 2026Article
- Methylation profiling in CNS tumor diagnostics: a single-centre real-world experience from Central Europe.Virchows Archiv : an international journal of pathology · 2026Article
- Nano-RAPID: Integrated Diagnosis for Sarcoma Using a Nanopore Sequencer.Genes, chromosomes & cancer · 2026Article
- Inflammatory Rhabdomyoblastic Tumor with Malignant Progression of the Neck: Unique Copy Number Profile Identified by Nanopore Sequencing.Head and neck pathology · 2026Article
- High clinical utility of comprehensive multi-omic molecular profiling of rare and hard-to-diagnose pediatric tumors.Research square · 2026Article
- Nodular Necrotizing Fibroblastic Sarcoma and Myxoinflammatory Fibroblastic Sarcoma: A Comparative Clinicopathological, Molecular, and Epigenetic Study.Genes, chromosomes & cancer · 2026Article
- Accurate Prediction of Central Conventional Chondrosarcoma Risk and Outcome Using Methylation Profiling: CHROME.Genes, chromosomes & cancer · 2026Article
- High clinical utility of comprehensive multi-omic molecular profiling of rare and hard-to-diagnose pediatric tumors.medRxiv : the preprint server for health sciences · 2026Article
- Integrating artificial intelligence into cancers of unknown primary diagnosis and treatment.iScience · 2026Review
- DNA methylation-based classification of hematolymphoid neoplasms.Blood advances · 2026Article
- Proposal of a Risk Stratification Algorithm for Extracranial Malignant Rhabdoid Tumors.Cancer medicine · 2026Article
- [Cutaneous clear cell sarcoma].Pathologie (Heidelberg, Germany) · 2026Review
- Integration and validation of complementary ex vivo assays for functional precision oncology.NPJ precision oncology · 2026Article
- The effect of neoadjuvant radiotherapy on immune cell infiltrates in myxofibrosarcoma.Immuno-oncology technology · 2026Article
- Radiotherapy Response Prediction in Myxofibrosarcomas and Undifferentiated Soft Tissue Sarcomas Using DNA Methylation and Copy Number Profiling.International journal of surgical pathology · 2026Article
233 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
111 authors at 20 institutions in 8 countries.
Christian Koelsche *Department of Neuropathology, Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany.ORCID 0000-0001-8763-8864
Daniel Schrimpf *Department of Neuropathology, Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany.
Damian Stichel *Clinical Cooperation Unit Neuropathology, German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ), Heidelberg, Germany.
Martin Sill *Hopp Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany.
Felix SahmDepartment of Neuropathology, Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany.ORCID 0000-0001-5441-1962
David E ReussDepartment of Neuropathology, Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany.
Mirjam BlattnerHopp Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany.
Barbara WorstHopp Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany.
Christoph E HeiligDivision of Translational Medical Oncology, National Center for Tumor Diseases (NCT) Heidelberg and German Cancer Research Center (DKFZ), German Cancer Consortium (DKTK), Heidelberg, Germany.
Katja BeckDivision of Translational Medical Oncology, National Center for Tumor Diseases (NCT) Heidelberg and German Cancer Research Center (DKFZ), German Cancer Consortium (DKTK), Heidelberg, Germany.
Peter HorakDivision of Translational Medical Oncology, National Center for Tumor Diseases (NCT) Heidelberg and German Cancer Research Center (DKFZ), German Cancer Consortium (DKTK), Heidelberg, Germany.ORCID 0000-0003-4536-9306
Simon KreutzfeldtDivision of Translational Medical Oncology, National Center for Tumor Diseases (NCT) Heidelberg and German Cancer Research Center (DKFZ), German Cancer Consortium (DKTK), Heidelberg, Germany.ORCID 0000-0003-3278-4586
Elke PaffHopp Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany.
Sebastian StarkHopp Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany.
Pascal JohannHopp Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany.
Florian SeltHopp Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany.
Jonas EckerHopp Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany.
Dominik SturmHopp Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany.ORCID 0000-0003-0250-1696
Kristian W PajtlerHopp Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany.
Annekathrin ReinhardtDepartment of Neuropathology, Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany.
Annika K WefersDepartment of Neuropathology, Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany.
Philipp SieversDepartment of Neuropathology, Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany.
Azadeh EbrahimiClinical Cooperation Unit Neuropathology, German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ), Heidelberg, Germany.
Abigail SuwalaDepartment of Neuropathology, Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany.
Francisco Fernández-KlettDepartment of Neuropathology, Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany.
Belén CasaliniClinical Cooperation Unit Neuropathology, German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ), Heidelberg, Germany.
Andrey KorshunovDepartment of Neuropathology, Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany.
Volker HovestadtBroad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID 0000-0002-3480-6649
Felix K F KommossDepartment of General Pathology, Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany.ORCID 0000-0003-0876-9087
Mark KriegsmannDepartment of General Pathology, Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany.
Matthias SchickGenomics and Proteomics Core Facility, German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ), Heidelberg, Germany.
Melanie Bewerunge-HudlerGenomics and Proteomics Core Facility, German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ), Heidelberg, Germany.ORCID 0000-0001-9038-7891
Till MildeHopp Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany.
Olaf WittHopp Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany.
Andreas E KulozikHopp Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany.ORCID 0000-0003-1953-0848
Marcel KoolHopp Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany.
Laura Romero-PérezMax-Eder Research Group for Pediatric Sarcoma Biology, Institute of Pathology, Faculty of Medicine, LMU Munich, Munich, Germany.ORCID 0000-0003-3196-4382
Thomas G P GrünewaldMax-Eder Research Group for Pediatric Sarcoma Biology, Institute of Pathology, Faculty of Medicine, LMU Munich, Munich, Germany.ORCID 0000-0003-0920-7377
Thomas KirchnerInstitute of Pathology, Faculty of Medicine, LMU Munich, Munich, Germany.
Wolfgang WickNeurology Clinic and National Center for Tumor Diseases, University Hospital Heidelberg, Heidelberg, Germany.ORCID 0000-0002-6171-634X
Michael PlattenDepartment of Neurology, Mannheim University Medical Center, University of Heidelberg, Mannheim, Germany.ORCID 0000-0002-4746-887X
Andreas UnterbergDepartment of Neurosurgery, Heidelberg University Hospital, Heidelberg, Germany.
Matthias UhlDepartment of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany.
Amir AbdollahiDepartment of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany.
Jürgen DebusDepartment of Radiation Oncology, Heidelberg University Hospital, Heidelberg, Germany.
Burkhard LehnerDepartment of Orthopaedics, Trauma Surgery and Paraplegiology, Heidelberg University Hospital, Heidelberg, Germany.
Christian ThomasInstitute of Neuropathology, University Hospital Münster, Münster, Germany.ORCID 0000-0002-6642-7774
Martin HasselblattInstitute of Neuropathology, University Hospital Münster, Münster, Germany.ORCID 0000-0003-2707-8484
Werner PaulusInstitute of Neuropathology, University Hospital Münster, Münster, Germany.
Christian HartmannDepartment of Neuropathology, Institute of Pathology, Hannover Medical School (MHH), Hannover, Germany.
Ori StaszewskiInstitute of Neuropathology, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Marco PrinzInstitute of Neuropathology, Faculty of Medicine, University of Freiburg, Freiburg, Germany.ORCID 0000-0002-0349-1955
Jürgen HenchDepartment of Neuropathology, Institute of Pathology, Basel University Hospital, Basel, Switzerland.
Stephan FrankDepartment of Neuropathology, Institute of Pathology, Basel University Hospital, Basel, Switzerland.ORCID 0000-0001-6165-0187
Yvonne M H Versleijen-JonkersDepartment of Medical Oncology, Radboud University Medical Center, Nijmegen, The Netherlands.ORCID 0000-0001-7623-0505
Marije E WeidemaDepartment of Medical Oncology, Radboud University Medical Center, Nijmegen, The Netherlands.
Thomas MentzelDermatopathology Bodensee, Friedrichshafen, Germany.
Klaus GriewankDepartment of Dermatology, University Hospital Essen, West German Cancer Center, University Duisburg-Essen, Essen, Germany.ORCID 0000-0003-3899-9449
Enrique de ÁlavaDepartment of Pathology, Institute of Biomedicine of Sevilla (IBiS), Virgen del Rocio University Hospital, CSIC/University of Sevilla/CIBERONC, Seville, Spain.
Juan Díaz MartínDepartment of Pathology, Institute of Biomedicine of Sevilla (IBiS), Virgen del Rocio University Hospital, CSIC/University of Sevilla/CIBERONC, Seville, Spain.ORCID 0000-0002-3985-6434
Miguel A Idoate GastearenaDepartment of Pathological Anatomy, Clínica Universidad de Navarra, University of Navarra, Pamplona, Spain.
Kenneth Tou-En ChangDepartment of Pathology and Laboratory Medicine, KK Women's and Children's Hospital, Singapore, Republic of Singapore.ORCID 0000-0001-5244-4285
Sharon Yin Yee LowDepartment of Neurosurgery, National Neuroscience Institute, Singapore, Republic of Singapore.
Adrian Cuevas-BourdierNational Center of Pathology (NCP), Laboratoire National de Santé (LNS), Dudelange, Luxembourg.
Michel MittelbronnNational Center of Pathology (NCP), Laboratoire National de Santé (LNS), Dudelange, Luxembourg.
Martin MynarekDepartment of Pediatric Hematology and Oncology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.ORCID 0000-0003-3302-2719
Stefan RutkowskiDepartment of Pediatric Hematology and Oncology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Ulrich SchüllerDepartment of Pediatric Hematology and Oncology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.ORCID 0000-0002-8731-1121
Viktor F MautnerDepartment of Neurology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Jens SchittenhelmDepartment of Neuropathology, Institute of Pathology and Neuropathology, University Hospital of Tübingen, Tübingen, Germany.ORCID 0000-0002-9168-6209
Jonathan SerranoDepartment of Pathology, New York University School of Medicine, New York, NY, USA.
Matija SnuderlDepartment of Pathology, New York University School of Medicine, New York, NY, USA.
Reinhard BüttnerInstitute of Pathology, Cologne University Hospital, Cologne, Germany.ORCID 0000-0001-8806-4786
Thomas KlingebielDepartment of Pediatric Hematology and Oncology, University Children's Hospital, Frankfurt/Main, Germany.ORCID 0000-0001-5726-9092
Rolf BusleiInstitute of Pathology, Sozialstiftung Bamberg, Klinikum am Bruderwald, Bamberg, Germany.
Manfred GesslerTheodor-Boveri-Institute/Biocenter, Developmental Biochemistry, Würzburg University, Würzburg, Germany.ORCID 0000-0002-7915-6045
Pieter WesselingPrincess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.
Winand N M DinjensDepartment of Pathology, Erasmus MC Cancer Institute, Rotterdam, The Netherlands.
Sebastian BrandnerDepartment of Neurodegeneration, Institute of Neurology, University College London, London, UK.ORCID 0000-0002-9821-0342
Zane JaunmuktaneDivision of Neuropathology, The National Hospital for Neurology and Neurosurgery, University College London Hospitals NHS Foundation Trust, London, UK.
Iben LyskjærResearch Department of Pathology, University College London, London, UK.
Peter SchirmacherDepartment of General Pathology, Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany.
Albrecht StenzingerDepartment of General Pathology, Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany.
Benedikt BrorsDivision of Applied Bioinformatics, German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ), Heidelberg, Germany.
Hanno GlimmTranslational and Functional Cancer Genomics, National Center for Tumor Diseases (NCT) and German Cancer Research Center (DKFZ), Heidelberg, Germany.
Christoph HeiningDepartment of Translational Medical Oncology, National Center for Tumor Diseases (NCT) Dresden and German Cancer Research Center (DKFZ), Dresden, Germany.
Oscar M TiradoSarcoma Research Group, Oncobell Program, Bellvitge Biomedical Research Institute (IDIBELL), CIBERONC, Barcelona, Catalonia, Spain.
Miguel Sáinz-JaspeadoSarcoma Research Group, Oncobell Program, Bellvitge Biomedical Research Institute (IDIBELL), CIBERONC, Barcelona, Catalonia, Spain.
Jaume MoraDepartment of Pediatric Onco-Hematology and Developmental Tumor Biology Laboratory, Hospital Sant Joan de Déu, Barcelona, Catalonia, Spain.
Javier AlonsoPediatric Solid Tumor Laboratory, Human Genetic Department, Research Institute of Rare Diseases, Instituto de Salud Carlos III (ISCIII), Madrid, Spain.ORCID 0000-0002-6287-8391
Xavier Garcia Del MuroMedical Oncology Service, Catalan Institute of Oncology (ICO), Bellvitge Biomedical Research Institute (IDIBELL), University of Barcelona, Barcelona, Catalonia, Spain.ORCID 0000-0001-5571-2847
Sebastian MoranCancer Epigenetics and Biology Program (PEBC), Bellvitge Biomedical Research Institute (IDIBELL), Barcelona, Catalonia, Spain.ORCID 0000-0003-4192-8983
Manel EstellerJosep Carreras Leukaemia Research Institute (IJC), Badalona, Barcelona, Catalonia, Spain.ORCID 0000-0003-4490-6093
Jamal K BenhamidaDepartment of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY, USA.ORCID 0000-0001-5542-9857
Marc LadanyiDepartment of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Eva WardelmannGerhard Domagk Institute of Pathology, University Hospital Münster, Münster, Germany.ORCID 0000-0001-6788-4910
Cristina AntonescuDepartment of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY, USA.ORCID 0000-0002-9717-8205
Adrienne FlanaganDepartment of Histopathology, Royal National Orthopaedic Hospital NHS Trust, Stanmore, Middlesex, UK.ORCID 0000-0002-2832-1303
Uta DirksenPediatrics III Pediatric Hematology, Oncology, Immunology, Cardiology, Pulmonology, West German Cancer Center, University Hospital Essen, Essen, Germany.ORCID 0000-0002-5435-7860
Peter HohenbergerDivision of Surgical Oncology and Thoracic Surgery, Mannheim University Medical Center, University of Heidelberg, Mannheim, Germany.ORCID 0000-0001-5359-5923
Daniel BaumhoerBone Tumour Reference Centre at the Institute of Pathology, University Hospital Basel and University of Basel, Basel, Switzerland.ORCID 0000-0002-2137-7507
Wolfgang HartmannDivision of Translational Pathology, Gerhard Domagk Institute of Pathology, University Hospital Münster, Münster, Germany.ORCID 0000-0002-7609-5021
Christian VokuhlDepartment of Pediatric Pathology, University Hospital of Schleswig-Holstein, Kiel, Germany.
Uta FluckeDepartment of Pathology, Radboud University Medical Center, Nijmegen, The Netherlands.
Iver PetersenInstitute of Pathology, SRH Poliklinik Gera GmbH, Gera, Germany.
Gunhild MechtersheimerDepartment of General Pathology, Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany.
David CapperDepartment of Neuropathology, Charité Universitätsmedizin Berlin, Berlin, Germany.ORCID 0000-0003-1945-497X
David T W JonesHopp Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany.
Stefan FröhlingDivision of Translational Medical Oncology, National Center for Tumor Diseases (NCT) Heidelberg and German Cancer Research Center (DKFZ), German Cancer Consortium (DKTK), Heidelberg, Germany.ORCID 0000-0001-7907-4595
Stefan M PfisterHopp Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany.
Andreas von DeimlingDepartment of Neuropathology, Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany. andreas.vondeimling@med.uni-heidelberg.de.ORCID 0000-0002-5863-540X
German Cancer Research Center · DEHeidelberg University · DEUniversity Hospital Münster · DEInstitut d'Investigació Biomédica de Bellvitge · ESUniversität Hamburg · DEMemorial Sloan Kettering Cancer Center · USRadboud University Nijmegen · NLInstituto de Biomedicina de Sevilla · ESLudwig-Maximilians-Universität München · DENew York University · USUniversity College London Hospitals NHS Foundation Trust · GBUniversity Hospital of Basel · CHUniversity of Freiburg · DEBroad Institute · USCharité - Universitätsmedizin Berlin · DEClinica Universidad de Navarra · ESDermatopathologie Friedrichshafen · DEErasmus MC Cancer Institute · NLGoethe University Frankfurt · DEHospital Sant Joan de Déu Barcelona · ES
Funding
X-RAY CRYSTALLOGRAPHYP30CA008748 · NCI · SLOAN-KETTERING INSTITUTE FOR CANCER RES · PI Nicholas D Socci · 1985 to 2026
$347.4MTargeting Oncogenic Pathways in Genetically Complex SarcomasP50CA217694 · NCI · SLOAN-KETTERING INST CAN RESEARCH · PI Marc Ladanyi · 2018 to 2026
$21.5MDepartment of Health BRC399/NS/RB/101410Medical Research CouncilNCI NIH HHS P30 CA008748NCI NIH HHS P50 CA217694
Abstract
Sarcomas are malignant soft tissue and bone tumours affecting adults, adolescents and children. They represent a morphologically heterogeneous class of tumours and some entities lack defining histopathological features. Therefore, the diagnosis of sarcomas is burdened with a high inter-observer variability and misclassification rate. Here, we demonstrate classification of soft tissue and bone tumours using a machine learning classifier algorithm based on array-generated DNA methylation data. This sarcoma classifier is trained using a dataset of 1077 methylation profiles from comprehensively pre-characterized cases comprising 62 tumour methylation classes constituting a broad range of soft tissue and bone sarcoma subtypes across the entire age spectrum. The performance is validated in a cohort of 428 sarcomatous tumours, of which 322 cases were classified by the sarcoma classifier. Our results demonstrate the potential of the DNA methylation-based sarcoma classification for research and future diagnostic applications.
Indexed as
AlgorithmsDNA MethylationMachine LearningBone NeoplasmsCohort StudiesDNA Copy Number VariationsHumansInternetReproducibility of ResultsSarcomaSensitivity and SpecificitySoft Tissue Neoplasms
Identifiers
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Read underepoch 390
Registered trials
NCT06140992ClinicalTrials.gov
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.