Evidence map›Paper›PMID 33479123›Full record

ArticleScience (New York, N.Y.)2021

JARID2 and AEBP2 regulate PRC2 in the presence of H2AK119ub1 and other histone modifications.

Vignesh Kasinath, Curtis Beck, Paul Sauer, Simon Poepsel, Jennifer Kosmatka, Marco Faini, Daniel Toso, Ruedi Aebersold, Eva Nogales

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science (New York, N.Y.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 149 papers.

0numbers the graph read from it
0cells of the map it votes in
149citing papers in PubMed
15.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

149 citing papers in PubMed, 251 citations in OpenAlex.

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89 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 3 countries.

Vignesh KasinathQB3 Institute, Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA. enogales@lbl.gov vigneshk@berkeley.edu.ORCID https://orcid.org/0000-0003-2686-0546
Curtis BeckDepartment of Molecular and Cellular Biology, University of California, Berkeley, CA, USA.ORCID https://orcid.org/0000-0002-6674-9802
Paul SauerQB3 Institute, Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.ORCID https://orcid.org/0000-0001-7204-5863
Simon PoepselUniversity of Cologne, Center for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital, Cologne, Germany.ORCID https://orcid.org/0000-0002-8304-4062
Jennifer KosmatkaDepartment of Molecular and Cellular Biology, University of California, Berkeley, CA, USA.ORCID https://orcid.org/0000-0001-5337-3298
Marco FainiDepartment of Biology, Institute of Molecular Systems Biology, ETH Zürich, Zürich, Switzerland.ORCID https://orcid.org/0000-0001-8131-7648
Daniel TosoQB3 Institute, Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
Ruedi AebersoldDepartment of Biology, Institute of Molecular Systems Biology, ETH Zürich, Zürich, Switzerland.ORCID https://orcid.org/0000-0002-9576-3267
Eva NogalesQB3 Institute, Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA. enogales@lbl.gov vigneshk@berkeley.edu.ORCID https://orcid.org/0000-0001-9816-3681
QB3 · USUniversity of California, Berkeley · USETH Zurich · CHUniversity of Cologne · DEUniversity of Zurich · CH

Funding

Structural studies of function and regulation of microtubules and transcriptional gene expression machineryR35GM127018 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Eva Nogales · 2018 to 2026
$4.3M
Howard Hughes Medical InstituteNIGMS NIH HHS R35 GM127018
6 · The paper itself

Abstract

Polycomb repressive complexes 1 and 2 (PRC1 and PRC2) cooperate to determine cell identity by epigenetic gene expression regulation. However, the mechanism of PRC2 recruitment by means of recognition of PRC1-mediated H2AK119ub1 remains poorly understood. Our PRC2 cryo-electron microscopy structure with cofactors JARID2 and AEBP2 bound to a H2AK119ub1-containing nucleosome reveals a bridge helix in EZH2 that connects the SET domain, H3 tail, and nucleosomal DNA. JARID2 and AEBP2 each interact with one ubiquitin and the H2A-H2B surface. JARID2 stimulates PRC2 through interactions with both the polycomb protein EED and the H2AK119-ubiquitin, whereas AEBP2 has an additional scaffolding role. The presence of these cofactors partially overcomes the inhibitory effect that H3K4me3 and H3K36me3 exert on core PRC2 (in the absence of cofactors). Our results support a key role for JARID2 and AEBP2 in the cross-talk between histone modifications and PRC2 activity.

Indexed as

Histone CodeAnimalsCryoelectron MicroscopyGene Expression RegulationHistonesHumansNucleosomesPolycomb Repressive Complex 2PR-SET DomainsRepressor ProteinsUbiquitinXenopusAEBP2 protein, humanhistone H3 trimethyl Lys4HistonesJARID2 protein, humanNucleosomesPolycomb Repressive Complex 2Repressor ProteinsUbiquitin

Identifiers

PMID33479123
PMCPMC7993630
OpenAlexW3125983721

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.