ArticleInternational journal of molecular sciences2021
Isolation and Characterization of Equine Uterine Extracellular Vesicles: A Comparative Methodological Study.
Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- Heat stress modulates extracellular vesicles miRNA cargo in bovine uterine fluid and uterine function.Frontiers in veterinary science · 2026Article
- Extracellular vesicles in bovine reproduction: their journey from gametogenesis to pregnancy.Frontiers in cell and developmental biology · 2026Review
- Divergent selection for litter size variability affects RNA cargo in oviductal extracellular vesicles related to embryonic development and survival.Biological research · 2025Article
- Dietary inulin supplementation in early gestation regulates uterine fluid exosomes and angiogenesis to improve embryo implantation in sows.Journal of animal science and biotechnology · 2025Article
- Extracellular Vesicles in Sport Horses: Potential Biomarkers and Modulators of Exercise Adaptation and Therapeutics.International journal of molecular sciences · 2025Review
- RNA profiles differ between small and large extracellular vesicle subsets isolated from porcine seminal plasma.BMC genomics · 2024Article
- Oviductal extracellular vesicles miRNA cargo varies in response to embryos and their quality.BMC genomics · 2024Article
- CNS cell-derived exosome signatures as blood-based biomarkers of neurodegenerative diseases.Frontiers in neuroscience · 2024Review
- Pro-Inflammatory Cytokine Priming and Purification Method Modulate the Impact of Exosomes Derived from Equine Bone Marrow Mesenchymal Stromal Cells on Equine Articular Chondrocytes.International journal of molecular sciences · 2023Article
- Isolation of Extracellular Vesicles from Human Follicular Fluid: Size-Exclusion Chromatography versus Ultracentrifugation.Biomolecules · 2023Article
- Article
- Extracellular Vesicles in Veterinary Medicine.Animals : an open access journal from MDPI · 2022Review
- Recent progress of interferon-tau research and potential direction beyond pregnancy recognition.The Journal of reproduction and development · 2022Review
- Uterine extracellular vesicles as multi-signal messengers during maternal recognition of pregnancy in the mare.Scientific reports · 2022Article
- Comparison of the Variability of Small Extracellular Vesicles Derived from Human Liver Cancer Tissues and Cultured from the Tissue Explants Based on a Simple Enrichment Method.Stem cell reviews and reports · 2022Article
- Extracellular vesicles in low volume uterine lavage and serum: novel and promising biomarker for endometritis in Arabian mares.BMC veterinary research · 2022Article
- Isolation of Extracellular Vesicles From the Bronchoalveolar Lavage Fluid of Healthy and Asthmatic Horses.Frontiers in veterinary science · 2022Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 4 countries.
Funding
Abstract
Extracellular vesicles (EVs) have been identified in the uterine fluid in different species and have been pointed as key players in the embryo-maternal dialogue, maternal recognition of pregnancy and establishment of pregnancy. However, little is known about the uterine EVs in the mare. Therefore, the present study aimed at characterizing EVs from uterine lavage of cyclic mares by comparing five EVs isolation methods and the combination of them: (1) ultracentrifugation (UC); (2) concentration of lavage volume by Centricon ultrafiltration (CE); (3) the use of CE with different washing steps (phosphate-buffered saline with or without trehalose); (4) size-exclusion chromatography with iZON-qEV columns, and (5) a combination of the methods with best results based on EVs yield, purity, and protein cargo profiles. Transmission electron microscopy and Western blotting confirmed the isolation of EVs by all methods but with quantitative and qualitative differences. Mass spectrometry provided differences in protein profiles between methods, number of identified proteins, and protein classes. Our results indicate that the combination of CE/trehalose/iZON/UC is an optimal method to isolate equine uterine EVs with good yield and purity that can be applied in future studies to determine the role of equine uterine EVs in embryo-maternal interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.