Evidence map›Paper›PMID 33475929›Full record

ArticleMolecular biology reports2021

Influence of microcurrent on the modulation of remodelling genes in a wound healing assay.

Daniela Fernanda Dezotti Silva, Lucas de Oliveira Fujii, Gabriela Bortolança Chiarotto, Camila Andrea de Oliveira, Thiago Antônio Moretti de Andrade, Alexandre Leite Rodrigues de Oliveira, Marcelo Augusto Marretto Esquisatto, Fernanda Aparecida Sampaio Mendonça, Gláucia Maria Tech Dos Santos, Andrea Aparecida de Aro

Abstract read
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In one paragraph

Article in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 57% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Daniela Fernanda Dezotti SilvaBiomedical Sciences Graduate Program, University Center of Herminio Ometto Foundation/FHO, Araras, São Paulo, Brazil.
Lucas de Oliveira FujiiBiomedical Sciences Graduate Program, University Center of Herminio Ometto Foundation/FHO, Araras, São Paulo, Brazil.
Gabriela Bortolança ChiarottoBiomedical Sciences Graduate Program, University Center of Herminio Ometto Foundation/FHO, Araras, São Paulo, Brazil.
Camila Andrea de OliveiraBiomedical Sciences Graduate Program, University Center of Herminio Ometto Foundation/FHO, Araras, São Paulo, Brazil.
Thiago Antônio Moretti de AndradeBiomedical Sciences Graduate Program, University Center of Herminio Ometto Foundation/FHO, Araras, São Paulo, Brazil.
Alexandre Leite Rodrigues de OliveiraDepartment of Structural and Functional Biology, Institute of Biology, State University of Campinas-UNICAMP, Campinas, São Paulo, Brazil.
Marcelo Augusto Marretto EsquisattoBiomedical Sciences Graduate Program, University Center of Herminio Ometto Foundation/FHO, Araras, São Paulo, Brazil.
Fernanda Aparecida Sampaio MendonçaBiomedical Sciences Graduate Program, University Center of Herminio Ometto Foundation/FHO, Araras, São Paulo, Brazil.
Gláucia Maria Tech Dos SantosBiomedical Sciences Graduate Program, University Center of Herminio Ometto Foundation/FHO, Araras, São Paulo, Brazil.
Andrea Aparecida de AroBiomedical Sciences Graduate Program, University Center of Herminio Ometto Foundation/FHO, Araras, São Paulo, Brazil. andreaaro80@gmail.com.ORCID http://orcid.org/0000-0003-2895-0000
Centro Universitário Herminio Ometto de Araras · BRUniversidade Estadual de Campinas (UNICAMP) · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The literature has shown the beneficial effects of microcurrent (MC) therapy on tissue repair. We investigated if the application of MC at 10 μA/90 s could modulate the expression of remodeling genes transforming growth factor beta (Tgfb), connective tissue growth factor (Ctgf), insulin-like growth factor 1 (Igf1), tenascin C (Tnc), Fibronectin (Fn1), Scleraxis (Scx), Fibromodulin (Fmod) and tenomodulin in NIH/3T3 fibroblasts in a wound healing assay. The cell migration was analyzed between days 0 and 4 in both fibroblasts (F) and fibroblasts + MC (F+MC) groups. On the 4th day, cell viability and gene expression were also analyzed after daily MC application. Higher expression of Ctgf and lower expression of Tnc and Fmod, respectively, were observed in the F+MC group in relation to F group (p < 0.05), and no difference was observed between the groups for the genes Tgfb, Fn1 and Scx. In cell migration, a higher number of cells in the scratch region was observed in group F+MC (p < 0.05) compared to group F on the 4th day, and the cell viability assay showed no difference between the groups. In conclusion, MC therapy at an intensity/time of 10 μA/90 s with 4 daily applications did not affect cell viability, stimulated fibroblasts migration with the involvement of Ctgf, and reduced the Tnc and Fmod expression.

Indexed as

Electric Stimulation TherapyAnimalsCell MovementConnective Tissue Growth FactorFibromodulinFibronectinsGene Expression RegulationHumansInsulin-Like Growth Factor IMiceNIH 3T3 CellsTenascinTransforming Growth Factor beta1Wound HealingCCN2 protein, mouseConnective Tissue Growth FactorFibromodulinFibronectinsFmod protein, mouseinsulin-like growth factor-1, mouseInsulin-Like Growth Factor ITenascinTgfb1 protein, mouseTnc protein, mouseTransforming Growth Factor beta1Cell migrationFibroblastsGenesMicrocurrentTherapy

Identifiers

PMID33475929
OpenAlexW3122784507

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.