ArticleThe Journal of general physiology2021
Phenanthrene impacts zebrafish cardiomyocyte excitability by inhibiting IKr and shortening action potential duration.
Article in The Journal of general physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 42 citations in OpenAlex.
- Subchronic PAH Exposure Impacts Cardiac Electrophysiology of the Polar Fish, Navaga Cod (Toxics · 2026Article
- Mathematical model of the zebrafish ventricular cardiomyocyte action potential and calcium transient.The Journal of physiology · 2026Article
- The air pollutant phenanthrene disrupts calcium cycling and alters repolarization in female sheep ventricular cardiomyocytes.Physiological reports · 2025Article
- The Effects of 3-Methylphenanthrene on Ion Currents Mediated by KBulletin of experimental biology and medicine · 2025Article
- Aryl hydrocarbon receptor-dependent toxicity by retene requires metabolic competence.Toxicological sciences : an official journal of the Society of Toxicology · 2024Article
- Integrating 4-D light-sheet fluorescence microscopy and genetic zebrafish system to investigate ambient pollutants-mediated toxicity.The Science of the total environment · 2023Review
- Global Air Pollutant Phenanthrene and Arrhythmic Outcomes in a Mouse Model.Environmental health perspectives · 2023Article
- Isolation of Cardiac and Vascular Smooth Muscle Cells from Adult, Juvenile, Larval and Embryonic Zebrafish for Electrophysiological Studies.Journal of visualized experiments : JoVE · 2022Article
- Inhibition of the hERG potassium channel by phenanthrene: a polycyclic aromatic hydrocarbon pollutant.Cellular and molecular life sciences : CMLS · 2021Article
- Phenanthrene alters the electrical activity of atrial and ventricular myocytes of a polar fish, the Navaga cod.Aquatic toxicology (Amsterdam, Netherlands) · 2021Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Air pollution is an environmental hazard that is associated with cardiovascular dysfunction. Phenanthrene is a three-ringed polyaromatic hydrocarbon that is a significant component of air pollution and crude oil and has been shown to cause cardiac dysfunction in marine fishes. We investigated the cardiotoxic effects of phenanthrene in zebrafish (Danio rerio), an animal model relevant to human cardiac electrophysiology, using whole-cell patch-clamp of ventricular cardiomyocytes. First, we show that phenanthrene significantly shortened action potential duration without altering resting membrane potential or upstroke velocity (dV/dt). L-type Ca2+ current was significantly decreased by phenanthrene, consistent with the decrease in action potential duration. Phenanthrene blocked the hERG orthologue (zfERG) native current, IKr, and accelerated IKr deactivation kinetics in a dose-dependent manner. Furthermore, we show that phenanthrene significantly inhibits the protective IKr current envelope, elicited by a paired ventricular AP-like command waveform protocol. Phenanthrene had no effect on other IK. These findings demonstrate that exposure to phenanthrene shortens action potential duration, which may reduce refractoriness and increase susceptibility to certain arrhythmia triggers, such as premature ventricular contractions. These data also reveal a previously unrecognized mechanism of polyaromatic hydrocarbon cardiotoxicity on zfERG by accelerating deactivation and decreasing IKr protective current.
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