ArticleThe world journal of men's health2021
Effects of Semen Processing on Sperm Function: Differences between Swim-Up and Density Gradient Centrifugation.
Article in The world journal of men's health, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06545318 (A Novel Sperm Selection Method Provides Advantages Over Conventional Approaches), which is not on this map. Cited by 13 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
A Novel Sperm Selection Method Provides Advantages Over Conventional Approaches: Validation Through a Clinical Pilot Study
Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Comparative analysis of sperm preparation techniques on DNA fragmentation and clinical outcomes: a network meta-analysis.Frontiers in endocrinology · 2026Pooled it
- Effect of Adding Human Umbilical Cord Mesenchymal Stem Cells-Derived Secretome on Sperm Quality Improvement by Swim-Up Method.Obstetrics and gynecology international · 2026Article
- Sperm selection for assisted reproductive technology: do we have a winner?Journal of assisted reproduction and genetics · 2025Review
- Functional parameters of spermatozoa obtained by a new selection device.FEBS open bio · 2025Article
- Sperm Separation and Selection Techniques to Mitigate Sperm DNA Damage.Life (Basel, Switzerland) · 2025Review
- Effect of Two Different Sperm Selection Methods on Boar Sperm Parameters and In Vitro Fertilisation Outcomes.Animals : an open access journal from MDPI · 2024Article
- Advanced Sperm Selection Techniques for Assisted Reproduction.Journal of personalized medicine · 2024Review
- Live motile sperm sorting device for enhanced sperm-fertilization competency: comparative analysis with density-gradient centrifugation and microfluidic sperm sorting.Journal of assisted reproduction and genetics · 2023Article
- Comparison between density gradient centrifugation method, an extended version of the horizontal swim up method and the combination of both for sperm selection.Obstetrics & gynecology science · 2023Article
- Analyzing the Differential Impact of Semen Preparation Methods on the Outcomes of Assisted Reproductive Techniques.Biomedicines · 2023Article
- The Effectiveness of Sperm Preparation Using Density Mini-Gradient and Single-Layer Centrifugation for Oligospermia Samples.Acta informatica medica : AIM : journal of the Society for Medical Informatics of Bosnia & Herzegovina : casopis Drustva za medicinsku informatiku BiH · 2022Article
- Cumulative live birth rates after IVF/ICSI cycles with sperm prepared by density gradient centrifugation vs. swim-up: a retrospective study using a propensity score-matching analysis.Reproductive biology and endocrinology : RB&E · 2022Article
- Sperm Selection for ICSI: Do We Have a Winner?Cells · 2021Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
purposeAndrology research has evolved notoriously in the latest years, particularly since male factor contribution to couple infertility has been undoubtedly demonstrated. However, sperm function investigations results are sometimes contradictory, probably as a result of the use of different sperm processing techniques. In this work, we underwent a systematic functional comparison of human sperm samples simultaneously processed by swim-up and density gradient centrifugation, which are the preferred sperm processing methods used in basic and clinical laboratories. MATERIALS AND
methodsTo compare functional characteristics of sperm isolated by swim-up and density gradient centrifugation followed by incubation at different times under capacitating conditions.
resultsSemen samples processed in parallel by these two procedures resulted in sperm preparations with significant differences in redox state, spontaneous intracellular calcium oscillations, hyperactivation, protein tyrosine phosphorylation, and acrosome reaction responsivity to calcium ionophore. Such differences showed time-dependent specific patterns for spontaneous intracellular calcium oscillations, hyperactivation and protein tyrosine phosphorylation. Sperm retrieved by density gradient centrifugation showed more hyperactivation and tyrosine phosphorylation than swim-up sperm, suggesting a higher degree of capacitation.
conclusionsOur results account for functional differences observed in spermatozoa processed with these two methods and therefore may contribute to a better interpretation of outcomes obtained in different laboratories as well as to improve experimental designs aimed to study sperm physiology and fertility potential.
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Registered trials
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