ArticleMitochondrial DNA. Part B, Resources2018
A rapid and efficient method for enriching mitochondrial DNA from plants.
Article in Mitochondrial DNA. Part B, Resources, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed.
- Multiple inducible DNA double-strand breaks using a restriction endonuclease in Arabidopsis thaliana mitochondria.BMC plant biology · 2026Article
- PMAT: an efficient plant mitogenome assembly toolkit using low-coverage HiFi sequencing data.Horticulture research · 2024Article
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3 authors.
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Abstract
Current mitochondrial purification techniques are tedious and protracted due to their emphasis on recovering physiologically active mitochondria. However, for studies that are exclusively interested in isolating mitochondrial DNA (mtDNA) for applications such as PCR and sequencing, respiring mitochondria - and the complex procedures that stem from the need to retain their function - are unnecessary. Still, global DNA extraction methods have proven insufficient for mitochondrial DNA isolation because nuclear mitochondrial DNA segments (NUMTs) pose unique challenges to accurate mtDNA quantification and characterization. We present a rapid and simple extraction technique that maximizes recovery of mitochondrial DNA from plant cells, while minimizing the presence of nuclear DNA. Through real-time PCR, we show that this method provides a significant increase in the enrichment of mitochondrial DNA compared to that of nuclear DNA in both
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