ArticleNature communications2021
Single-cell analysis of Schistosoma mansoni identifies a conserved genetic program controlling germline stem cell fate.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 65 citations in OpenAlex.
- Flexible use of conserved motifs constrains genome access in cell type evolution.Nature ecology & evolution · 2026Article
- Article
- Single-cell transcriptomics identifies a p21-activated kinase important for survival of the zoonotic parasiteiScience · 2026Article
- Transcriptome profiling of intra-snail stages of the liver fluke Fasciola hepatica reveals key mediators underlying parasite development and interaction with the host.BMC genomics · 2026Article
- A male-pheromone-elevated transcription factor ZNF362.1 in female schistosomes determines sexual maturation.Science advances · 2026Article
- Schistosome esophageal gland factor MEG-8.2 drives host cell lysis and interacts with host immune proteins.PLoS pathogens · 2026Article
- Dynamic single-cell transcriptomics reveals lsamp-guided neural network formation in male S. japonicum driving female reproduction.Nature communications · 2026Article
- CRISPR/Cas genome editing, functional genomics, and diagnostics for parasitic helminths.International journal for parasitology · 2025Review
- REVIEW: THE ORIGIN OF DIGENETIC TREMATODES: DID THE STORY BEGIN WITH THE BLOOD FLUKES?The Journal of parasitology · 2025Review
- The retinoic acid family-like nuclear receptor SmRAR identified by single-cell transcriptomics of ovarian cells controls oocyte differentiation in Schistosoma mansoni.Nucleic acids research · 2025Article
- The DEAD-box RNA helicase eIF4A is a crucial factor for stem-cell activity and reproduction of the parasiteFrontiers in cellular and infection microbiology · 2025Article
- A single-cell atlas of the miracidium larva ofeLife · 2024Article
- Integrating phylogenies into single-cell RNA sequencing analysis allows comparisons across species, genes, and cells.PLoS biology · 2024Article
- Intestinal cell diversity and treatment responses in a parasitic nematode at single cell resolution.BMC genomics · 2024Article
- Single-cell transcriptomics of the human parasite Schistosoma mansoni first intra-molluscan stage reveals tentative tegumental and stem-cell regulators.Scientific reports · 2024Article
- Rhodopsin orphan GPCR20 interacts with neuropeptides and directs growth, sexual differentiation, and egg production in femaleMicrobiology spectrum · 2024Article
- Schistosomiasis.Advances in experimental medicine and biology · 2024Review
- Genome-wide transcriptome analysis ofFrontiers in cellular and infection microbiology · 2024Article
- Trematode Genomics and Proteomics.Advances in experimental medicine and biology · 2024Article
- Purification and transcriptomic characterization of proliferative cells ofFrontiers in parasitology · 2024Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Schistosomes are parasitic flatworms causing one of the most prevalent infectious diseases from which millions of people are currently suffering. These parasites have high fecundity and their eggs are both the transmissible agents and the cause of the infection-associated pathology. Given its biomedical significance, the schistosome germline has been a research focus for more than a century. Nonetheless, molecular mechanisms that regulate its development are only now being understood. In particular, it is unknown what balances the fate of germline stem cells (GSCs) in producing daughter stem cells through mitotic divisions versus gametes through meiosis. Here, we perform single-cell RNA sequencing on juvenile schistosomes and capture GSCs during de novo gonadal development. We identify a genetic program that controls the proliferation and differentiation of GSCs. This program centers around onecut, a homeobox transcription factor, and boule, an mRNA binding protein. Their expressions are mutually dependent in the schistosome male germline, and knocking down either of them causes over-proliferation of GSCs and blocks germ cell differentiation. We further show that this germline-specific regulatory program is conserved in the planarian, schistosome's free-living evolutionary cousin, but the function of onecut has changed during evolution to support GSC maintenance.
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