Evidence map›Paper›PMID 33472649›Full record

ArticleInfectious agents and cancer2021

Molecular detection of urogenital mollicutes in patients with invasive malignant prostate tumor.

Osama Mohammed Saed Abdul-Wahab, Mishari H Al-Shyarba, Boutheina Ben Abdelmoumen Mardassi, Nessrine Sassi, Majed Saad Shaya Al Fayi, Hassan Otifi, Abdullah Hassan Al Murea, Béhija Mlik, Elhem Yacoub

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Article in Infectious agents and cancer, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
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  6. The Relationship betweenJournal of oncology · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Osama Mohammed Saed Abdul-WahabDepartment of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia.
Mishari H Al-ShyarbaDepartment of Surgery, College of Medicine, King Khalid University, P.O. Box 641, Abha, 61421, Saudi Arabia.
Boutheina Ben Abdelmoumen MardassiSpecialized Unit of Mycoplasmas, Laboratory of Molecular Microbiology, Vaccinology, and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El-Manar, Tunis, Tunisia.
Nessrine SassiSpecialized Unit of Mycoplasmas, Laboratory of Molecular Microbiology, Vaccinology, and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El-Manar, Tunis, Tunisia.
Majed Saad Shaya Al FayiDepartment of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, King Khalid University, P.O. Box 641, Abha, 61421, Saudi Arabia.
Hassan OtifiDepartment of Pathology, College of Medicine, King Khalid University, P.O. Box 641, Abha, 61421, Saudi Arabia.
Abdullah Hassan Al MureaLaboratory Department, Aseer Central Hospital, P. O. Box 34, Abha, 51411, Saudi Arabia.
Béhija MlikSpecialized Unit of Mycoplasmas, Laboratory of Molecular Microbiology, Vaccinology, and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El-Manar, Tunis, Tunisia.
Elhem YacoubSpecialized Unit of Mycoplasmas, Laboratory of Molecular Microbiology, Vaccinology, and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El-Manar, Tunis, Tunisia. elhem.yacoub@pasteur.utm.tn.ORCID http://orcid.org/0000-0003-2994-4622

Funding

King Khalid University G.R.P1/39/39
6 · The paper itself

Abstract

backgroundThe etiology of prostate cancer (PCa) is multiple and complex. Among the causes recently cited are chronic infections engendered by microorganisms that often go unnoticed. A typical illustration of such a case is infection due to mollicutes bacteria. Generally known by their lurking nature, urogenital mollicutes are the most incriminated in PCa. This study was thus carried out in an attempt to establish the presence of these mollicutes by PCR in biopsies of confirmed PCa patients and to evaluate their prevalence.

methodsA total of 105 Formalin-Fixed Paraffin-Embedded prostate tissues collected from 50 patients suffering from PCa and 55 with benign prostate hyperplasia were subjected to PCR amplification targeting species-specific genes of 5 urogenital mollicutes species, Mycoplasma genitalium, M. hominis, M. fermentans, Ureaplasma parvum, and U. urealyticum. PCR products were then sequenced to confirm species identification. Results significance was statistically assessed using Chi-square and Odds ratio tests.

resultsPCR amplification showed no positive results for M. genitalium, M. hominis, and M. fermentans in all tested patients. Strikingly, Ureaplasma spp. were detected among 30% (15/50) of PCa patients. Nucleotide sequencing further confirmed the identified ureaplasma species, which were distributed as follows: 7 individuals with only U. parvum, 5 with only U. urealyticum, and 3 co-infection cases. Association of the two ureaplasma species with PCa cases proved statistically significant (P < 0.05), and found to represent a risk factor. Of note, Ureaplasma spp. were mostly identified in patients aged 60 and above with prostatic specific antigen (PSA) level > 4 ng/ml and an invasive malignant prostate tumor (Gleason score 8-10).

conclusionsThis study uncovered a significant association of Ureaplasma spp. with PCa arguing in favour of their potential involvement in this condition. Yet, this finding, though statistically supported, warrants a thorough investigation at a much larger scale.

Indexed as

PCRProstate cancerSequencingUrogenital mollicutes

Identifiers

PMID33472649
PMCPMC7816065

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.