ArticleBMC biotechnology2021
Comparing various protocols of human and bovine ovarian tissue decellularization to prepare extracellular matrix-alginate scaffold for better follicle development in vitro.
Article in BMC biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.
- Bioengineering trends in female reproduction: a systematic review.Human reproduction update · 2022Pooled it
- The matrisome, methods, analytical evaluation and potential of decellularized porcine ovaries for fertility preservation.Cell and tissue research · 2026Review
- Decellularized ovarian bioscaffolds and resveratrol-loaded polymeric nanoparticles support in vitro viability and ultrastructural preservation of bovine preantral follicles.Journal of assisted reproduction and genetics · 2026Article
- Isorhamnetin-preconditioned MSC-derived exosomes restore ovarian function by inhibiting ferroptosis in chemotherapy-induced POF.Stem cell research & therapy · 2026Article
- MACS and acellular autologous non-ovarian tissue scaffolds: a promising strategy for safe and efficient follicle transplantation in hematologic cancer.Journal of ovarian research · 2025Article
- Preparation and characterization of human decellularized ovarian scaffold based on supercritical carbon dioxide protocol.Biomedical engineering online · 2025Article
- Optimizations of Placenta Extracellular Matrix-Loaded Silk Fibroin/Alginate 3D-Printed Scaffolds Structurally and Functionally for Bone Tissue Engineering.Engineering in life sciences · 2025Article
- Research advances in the construction of stem cell-derived ovarian organoids.Stem cell research & therapy · 2024Review
- Decellularization of various tissues and organs through chemical methods.Tissue & cell · 2024Review
- A spotlight on factors influencing the in vitro folliculogenesis of isolated preantral follicles.Journal of assisted reproduction and genetics · 2024Article
- 3D morphometry of endothelial cells angiogenesis in an extracellular matrix composite hydrogel.Heliyon · 2024Article
- Enhancing organoid culture: harnessing the potential of decellularized extracellular matrix hydrogels for mimicking microenvironments.Journal of biomedical science · 2024Review
- Characterization of Acellular Cartilage Matrix-Sodium Alginate Scaffolds in Various Proportions.Tissue engineering. Part C, Methods · 2024Article
- Reconstruction of ovarian follicular-like structure by recellularization of a cell-free human ovarian scaffold with mouse fetal ovarian cells.Cytotechnology · 2024Article
- Method of Isolation and In Vitro Culture of Primordial Follicles in Bovine Animal Model.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The stromal microenvironment and ovarian aging: mechanisms and therapeutic opportunities.Journal of ovarian research · 2023Review
- The Decellularized Calf Testis: Introducing Suitable Scaffolds for Spermatogenesis Studies.International journal of fertility & sterility · 2023Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNowadays, the number of cancer survivors is significantly increasing as a result of efficient chemo/radio therapeutic treatments. Female cancer survivors may suffer from decreased fertility. In this regard, different fertility preservation techniques were developed. Artificial ovary is one of these methods suggested by several scientific groups. Decellularized ovarian cortex has been introduced as a scaffold in the field of human fertility preservation. This study was carried out to compare decellularization of the ovarian scaffold by various protocols and evaluate the follicle survival in extracellular matrix (ECM)-alginate scaffold.
resultsThe micrographs of H&E and DAPI staining confirmed successful decellularization of the ovarian cortex in all experimental groups, but residual DNA content in SDS-Triton group was significantly higher than other groups (P < 0.05). SEM images demonstrated that complex fiber network and porosity structure were maintained in all groups. Furthermore, elastin and collagen fibers were observed in all groups after decellularization process. MTT test revealed higher cytobiocompatibility of the SDS-Triton-Ammonium and SDS-Triton decellularized scaffolds compared with SDS groups. Compared to the transferred follicles into the sodium alginate (81%), 85.9% of the transferred follicles into the decellularized scaffold were viable after 7 days of cultivation (P = 0.04).
conclusionAlthough all the decellularization procedures was effective in removal of cells from ovarian cortex, SDS-Triton-Ammonium group showed less residual DNA content with higher cytobiocompatibility for follicles when compared with other groups. In addition, the scaffold made from ovarian tissues decellularized using SDS-Triton-Ammonium and sodium alginate is suggested as a potential 3D substrate for in vitro culture of follicles for fertility preservation.
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