Evidence map›Paper›PMID 33471936›Full record

ArticleJournal of veterinary internal medicine2021

Characterization of the intestinal mucosal proteome in cats with inflammatory bowel disease and alimentary small cell lymphoma.

Sina Marsilio, Floris C Dröes, Lawrence Dangott, Betty Chow, Steve Hill, Mark Ackermann, J Scott Estep, Jonathan A Lidbury, Jan S Suchodolski, Jörg M Steiner

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of veterinary internal medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.1field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 1 country.

Sina MarsilioDepartment of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA, USA.ORCID https://orcid.org/0000-0002-0693-0669
Floris C DröesGastrointestinal Laboratory, Department of Small Animal Clinical Sciences, Texas A&M College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, USA.
Lawrence DangottProtein Chemistry Laboratory, Department of Biochemistry & Biophysics, Texas A&M University, College Station, Texas, USA.
Betty ChowVeterinary Specialty Hospital, San Diego, California, USA.ORCID https://orcid.org/0000-0002-7775-3719
Steve HillVeterinary Specialty Hospital, San Diego, California, USA.
Mark AckermannOregon Veterinary Diagnostic Laboratory, Carlson College of Veterinary Medicine, Oregon State University, Corvallis, Oregon, USA.
J Scott EstepTexas Veterinary Pathology, LLC, San Antonio, Texas, USA.
Jonathan A LidburyGastrointestinal Laboratory, Department of Small Animal Clinical Sciences, Texas A&M College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, USA.ORCID https://orcid.org/0000-0001-5107-4577
Jan S SuchodolskiGastrointestinal Laboratory, Department of Small Animal Clinical Sciences, Texas A&M College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, USA.ORCID https://orcid.org/0000-0002-2176-6932
Jörg M SteinerGastrointestinal Laboratory, Department of Small Animal Clinical Sciences, Texas A&M College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, USA.ORCID https://orcid.org/0000-0003-3336-2086
Texas College · USOrthopedic Specialty Hospital · USOregon State University · USTexas A&M University · USUrology San Antonio · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCurrent tests for diagnosis and differentiation of lymphoplasmacytic enteritis (LPE) and small cell lymphoma (SCL) in cats are expensive, invasive, and lack specificity. The identification of less invasive, more reliable biomarkers would facilitate diagnosis.

objectivesTo characterize the mucosal proteome in endoscopically obtained, small intestinal tissue biopsy specimens. We hypothesized that differentially expressed proteins could be identified and serve as biomarker candidates for the differentiation of LPE and SCL in cats. ANIMALS: Six healthy control cats, 6 cats with LPE, and 8 cats with SCL.

methodsThe mucosal proteome was analyzed using 2-dimensional fluorescence difference gel electrophoresis (2D DIGE) and nanoflow liquid chromatography tandem mass spectrometry. For 5 proteins, results were verified by Western blot analysis.

resultsA total of 2349 spots were identified, of which 9 were differentially expressed with a ≥2-fold change between healthy cats and cats with LPE and SCL (.01 < P < .001). Eight of these 9 spots were also differentially expressed between cats with LPE and cats with SCL (P .001 < P < .04). However, Western blot analysis for malate dehydrogenase-1, malate dehydrogenase-2, apolipoprotein, annexin IV, and annexin V did not confirm significant differential protein expression for any of the 5 proteins assessed. CONCLUSIONS AND CLINICAL IMPORTANCE: Two-D DIGE did not identify potential biomarker candidates in the intestinal mucosa of cats with LPE and SCL. Future studies should focus on different techniques to identify biomarker candidates for cats with chronic enteropathies (CE).

Indexed as

Inflammatory Bowel DiseasesLeukemia, Lymphocytic, Chronic, B-CellLymphoma, Non-HodgkinAnimalsIntestinal MucosaProteomeProteomeEATLenteropathy-associated T-cell lymphomafeline chronic enteropathy

Identifiers

PMID33471936
PMCPMC7848303
OpenAlexW3124364505

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.