ArticlePloS one2021
A universal RT-qPCR assay for "One Health" detection of influenza A viruses.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 81 citations in OpenAlex.
- Investigating high pathogenicity avian influenza virus incursions to remote islands: detection of H5N1 on Gough Island in the South Atlantic Ocean.Emerging microbes & infections · 2026Article
- Rab11Bis required for binding and entry of recent H3N2, but not H1N1, influenza A isolates.Journal of virology · 2026Article
- Comparative analysis of laboratory-based and portable qPCR platforms: CFX, MIC, and Biomeme Franklin for African swine fever and avian influenza detection.BMC veterinary research · 2026Article
- Novel avian papillomaviruses identified in a south polar skua sampled on Ross Island, Antarctica.Archives of virology · 2026Article
- Concurrent Detection of Swine-Origin Influenza A(H1N1) Virus in Pigs and Farmer, Switzerland.Emerging infectious diseases · 2026Article
- Epidemiology of Influenza A virus in Swiss pig herds: subclinical circulation and associated risk factors.Porcine health management · 2026Article
- Diverse genomic landscape of swine influenza A virus in England (2014-2021).Microbial genomics · 2026Article
- Detection of Two High Pathogenicity Avian Influenza Virus (HPAIV) Subtypes, H5N1 and H5N5, in a Mass Mortality Event in Wild Seabirds and Co-Location With Dead Seals.Transboundary and emerging diseases · 2026Article
- Genomic-based biosurveillance for avian influenza: whole genome sequencing from wild mallards sampled during autumn migration in 2022-2023 reveals a high co-infection rate on migration stopover site in Georgia.Frontiers in microbiology · 2026Article
- Quantifying iris pigmentation irregularities following an avian influenza outbreak: implications for population-level disease surveillance in a colonial seabird.Conservation physiology · 2026Article
- Rapid surge of reassortant A(H1N1) influenza viruses in Danish swine and their zoonotic potential.Emerging microbes & infections · 2025Article
- Detection of clade 2.3.4.4b H5N1 high pathogenicity avian influenza virus in a sheep in Great Britain, 2025.Emerging microbes & infections · 2025Article
- Rab11B is required for binding and entry of recent H3N2, but not H1N1, influenza A isolates.bioRxiv : the preprint server for biology · 2025Article
- Natural infections of highly pathogenic avian influenza virus H5N1 in wild birds between 2020 and 2023 in the UK: a retrospective study with focus on microscopic lesions, viral distribution and neurotropism.Veterinary research · 2025Article
- Primary care sentinel surveillance of influenza-like illness and laboratory detections of respiratory viral infections in Denmark, week 40 2021 to week 39 2023.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2025Article
- Optimized high-throughput whole-genome sequencing workflow for surveillance of influenza A virus.Genome medicine · 2025Article
- Investigating factors driving shifts in subtype dominance within H5Nx clade 2.3.4.4b high pathogenicity avian influenza viruses.The Journal of general virology · 2025Article
- Monitoring influenza A virus in wild migratory birds and waterfowl in Libya using RT-qPCR.Open veterinary journal · 2025Article
- Asymptomatic infection and antibody prevalence to co-occurring avian influenza viruses vary substantially between sympatric seabird species following H5N1 outbreaks.Scientific reports · 2025Article
- Wild passerines as potential carriers and sources of avian influenza viruses in Ukraine.Frontiers in microbiology · 2025Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mutual dependence of human and animal health is central to the One Health initiative as an integrated strategy for infectious disease control and management. A crucial element of the One Health includes preparation and response to influenza A virus (IAV) threats at the human-animal interface. The IAVs are characterized by extensive genetic variability, they circulate among different hosts and can establish host-specific lineages. The four main hosts are: avian, swine, human and equine, with occasional transmission to other mammalian species. The host diversity is mirrored in the range of the RT-qPCR assays for IAV detection. Different assays are recommended by the responsible health authorities for generic IAV detection in birds, swine or humans. In order to unify IAV monitoring in different hosts and apply the One Health approach, we developed a single RT-qPCR assay for universal detection of all IAVs of all subtypes, species origin and global distribution. The assay design was centred on a highly conserved region of the IAV matrix protein (MP)-segment identified by a comprehensive analysis of 99,353 sequences. The reaction parameters were effectively optimised with efficiency of 93-97% and LOD95% of approximately ten IAV templates per reaction. The assay showed high repeatability, reproducibility and robustness. The extensive in silico evaluation demonstrated high inclusivity, i.e. perfect sequence match in the primers and probe binding regions, established as 94.6% for swine, 98.2% for avian and 100% for human H3N2, pandemic H1N1, as well as other IAV strains, resulting in an overall predicted detection rate of 99% on the analysed dataset. The theoretical predictions were confirmed and extensively validated by collaboration between six veterinary or human diagnostic laboratories on a total of 1970 specimens, of which 1455 were clinical and included a diverse panel of IAV strains.
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