ArticleAnalytical chemistry2021
Suitcase Lab for Rapid Detection of SARS-CoV-2 Based on Recombinase Polymerase Amplification Assay.
Article in Analytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
62 citing papers in PubMed, 109 citations in OpenAlex.
- A multi-country phase 2 study to evaluate the suitcase lab for rapid detection of SARS-CoV-2 in seven Sub-Saharan African countries: Lessons from the field.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2023Trial
- An ultrafast SARS-CoV-2 virus enrichment and extraction method compatible with multiple modalities for RNA detection.Analytica chimica acta · 2021Trial
- Development and pilot application of a point-of-need molecular xenomonitoring protocol for tsetse (Glossina sp.) in a low-resource setting.PLoS neglected tropical diseases · 2026Article
- Diagnostic tests for urinary tract infections and antimicrobial resistance-a reality check between diagnostic innovation and implementation: a critical narrative review.Frontiers in cellular and infection microbiology · 2026Review
- Article
- Field Evaluation of Mobile Molecular Differential Tests in DRC and Nigeria.Open forum infectious diseases · 2025Article
- Development of a novel, rapid, and accurate recombinase polymerase amplification assay for the identification of the glassy-winged sharpshooter,3 Biotech · 2025Article
- Rapid Detection Assay for Infectious Bronchitis Virus Using Real-Time Reverse Transcription Recombinase-Aided Amplification.Viruses · 2025Article
- SIMPLE: A One-Pot RPA-Cas12b Method for Single Nucleotide Variations Identification with an Expanded Scope and Clinical Applications.Phenomics (Cham, Switzerland) · 2025Article
- Electrochemical Impedance Spectroscopy-Based Biosensors for Label-Free Detection of Pathogens.Biosensors · 2025Review
- Advances in the recombinase polymerase amplification platform for urogenital schistosomiasis diagnosis (ShDraI-RPA) towards implementation at the point-of-care.Scientific reports · 2025Article
- An open source, PCR based, point-of-care testing platform.Scientific reports · 2025Article
- Developing, Characterizing, and Modeling CRISPR-Based Point-of-Use Pathogen Diagnostics.ACS synthetic biology · 2025Article
- Rapid, sensitive and multiplexed detection of SARS-CoV-2 viral nucleic acids enabled by phase-based surface plasmon resonance of metallic gratings.Biomedical optics express · 2024Article
- Evaluation and comparison of one-step real-time PCR and one-step RT-LAMP methods for detection of SARS-CoV-2.BMC infectious diseases · 2024Article
- Developing, characterizing and modeling CRISPR-based point-of-use pathogen diagnostics.bioRxiv : the preprint server for biology · 2024Article
- Development of RPA-Cas12a-fluorescence assay for rapid and reliable detection of human bocavirus 1.Animal models and experimental medicine · 2024Article
- Article
- A rapid and visual detection assay for Senecavirus A based on recombinase-aided amplification and lateral flow dipstick.Frontiers in cellular and infection microbiology · 2024Article
- Addressing the challenges of establishing quality wastewater or non-sewered sanitation-based surveillance, including laboratory and epidemiological considerations, in Malawi.BMJ global health · 2023Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
27 authors at 9 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In March 2020, the SARS-CoV-2 virus outbreak was declared as a world pandemic by the World Health Organization (WHO). The only measures for controlling the outbreak are testing and isolation of infected cases. Molecular real-time polymerase chain reaction (PCR) assays are very sensitive but require highly equipped laboratories and well-trained personnel. In this study, a rapid point-of-need detection method was developed to detect the RNA-dependent RNA polymerase (RdRP), envelope protein (E), and nucleocapsid protein (N) genes of SARS-CoV-2 based on the reverse transcription recombinase polymerase amplification (RT-RPA) assay. RdRP, E, and N RT-RPA assays required approximately 15 min to amplify 2, 15, and 15 RNA molecules of molecular standard/reaction, respectively. RdRP and E RT-RPA assays detected SARS-CoV-1 and 2 genomic RNA, whereas the N RT-RPA assay identified only SARS-CoV-2 RNA. All established assays did not cross-react with nucleic acids of other respiratory pathogens. The RT-RPA assay's clinical sensitivity and specificity in comparison to real-time RT-PCR (
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.