ArticlePlant biotechnology journal2021
Rational design and testing of abiotic stress-inducible synthetic promoters from poplar cis-regulatory elements.
Article in Plant biotechnology journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 49 citations in OpenAlex.
- Elicitation and scale-up of Bacopa saponin production by plant cell suspension culture in stirred tank bioreactor.Bioprocess and biosystems engineering · 2026Article
- Design and Testing of Root-Specific Synthetic Promoters by Machine Learning.International journal of molecular sciences · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Induction of Embryogenic Callus, Protoplast Isolation, and PEG-Mediated Transformation Protocols inPlants (Basel, Switzerland) · 2026Article
- Plant responses to cold stress: molecular and physiological adaptive mechanisms with breeding advances.Frontiers in plant science · 2026Review
- Identification and application of bioparts for plant synthetic biology.Molecules and cells · 2025Review
- Article
- A comprehensive review of the transcriptomic and metabolic responses of grapevines to arbuscular mycorrhizal fungi.Planta · 2025Review
- Recent advances in designing synthetic plant regulatory modules.Frontiers in plant science · 2025Review
- Promoters, KeyInternational journal of molecular sciences · 2024Review
- Promoter of CassavaInternational journal of molecular sciences · 2024Article
- Design and synthesis of synthetic promoters for consistency of gene expression across growth phases and scale inSynthetic and systems biotechnology · 2024Article
- Novel synthetic inducible promoters controlling gene expression during water-deficit stress with green tissue specificity in transgenic poplar.Plant biotechnology journal · 2024Article
- Drought stress-inducible synthetic promoters designed for poplar are functional in rice.Plant cell reports · 2024Article
- Plant Engineering to Enable Platforms for Sustainable Bioproduction of Terpenoids.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Analysis of plant pararetrovirus promoter sequence(s) for developing a useful synthetic promoter with enhanced activity in rice, pearl millet, and tobacco plants.Frontiers in plant science · 2024Article
- Development of robust constitutive synthetic promoter using genetic resources of plant pararetroviruses.Frontiers in plant science · 2024Article
- Synthetic Promoter Design and Functional Evaluation in Saccharomyces cerevisiae.Methods in molecular biology (Clifton, N.J.) · 2024Article
- From qualitative to quantitative: the state of the art and challenges for plant synthetic biology.Quantitative biology (Beijing, China) · 2023Review
- Article
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9 authors at 5 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Abiotic stress resistance traits may be especially crucial for sustainable production of bioenergy tree crops. Here, we show the performance of a set of rationally designed osmotic-related and salt stress-inducible synthetic promoters for use in hybrid poplar. De novo motif-detecting algorithms yielded 30 water-deficit (SD) and 34 salt stress (SS) candidate DNA motifs from relevant poplar transcriptomes. We selected three conserved water-deficit stress motifs (SD18, SD13 and SD9) found in 16 co-expressed gene promoters, and we discovered a well-conserved motif for salt response (SS16). We characterized several native poplar stress-inducible promoters to enable comparisons with our synthetic promoters. Fifteen synthetic promoters were designed using various SD and SS subdomains, in which heptameric repeats of five-to-eight subdomain bases were fused to a common core promoter downstream, which, in turn, drove a green fluorescent protein (GFP) gene for reporter assays. These 15 synthetic promoters were screened by transient expression assays in poplar leaf mesophyll protoplasts and agroinfiltrated Nicotiana benthamiana leaves under osmotic stress conditions. Twelve synthetic promoters were induced in transient expression assays with a GFP readout. Of these, five promoters (SD18-1, SD9-2, SS16-1, SS16-2 and SS16-3) endowed higher inducibility under osmotic stress conditions than native promoters. These five synthetic promoters were stably transformed into Arabidopsis thaliana to study inducibility in whole plants. Herein, SD18-1 and SD9-2 were induced by water-deficit stress, whereas SS16-1, SS16-2 and SS16-3 were induced by salt stress. The synthetic biology design pipeline resulted in five synthetic promoters that outperformed endogenous promoters in transgenic plants.
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