ArticleMethods in molecular biology (Clifton, N.J.)2021
Selection of Suitable Reference Genes for qRT-PCR Gene Expression Studies in Rice.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Article
- Selection and validation of reference genes for quantitative real-time PCR during the developmental stages of seeds inFrontiers in plant science · 2025Article
- Evaluation and validation of suitable reference genes for quantitative real-time PCR analysis in lotus (Nelumbo nucifera Gaertn.).Scientific reports · 2024Article
- Genome-Wide Identification and Characterization of theInternational journal of molecular sciences · 2024Article
- Article
- Identification and validation of reference genes in vetiver (Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Non-targeted Metabolomics Reveals Metabolic Characteristics of Porcine Atretic Follicles.Frontiers in veterinary science · 2021Article
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2 authors.
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Abstract
With a widely established use of quantitative real-time PCR (qRT-PCR) for gene expression analysis, reliable and stable expression of reference genes is often discussed. Suitable reference genes should show less variation of expression across the target samples and allow for error minimization by normalization of qRT-PCR data. Therefore, selection of reliable reference genes is essential for accurate results and to support the conclusions drawn on expression levels of genes under study. In this chapter, we describe the workflow for selection and evaluation of reference genes in rice, including identification of candidate genes by using Genevestigator
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