Evidence map›Paper›PMID 33471322›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

TransGene Stacking II Vector System for Plant Metabolic Engineering and Synthetic Biology.

Qinlong Zhu, Yao-Guang Liu

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Qinlong ZhuState Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, South China Agricultural University, Guangzhou, China.
Yao-Guang LiuState Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, South China Agricultural University, Guangzhou, China. ygliu@scau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Efficient stacking of multiple genes is a critical element in metabolic engineering of complex pathways, synthetic biology, and genetic improvement of complex agronomic traits in plants. Here we present a high-efficiency multigene assembly and transformation vector system, TransGene Stacking II (TGS II), for these purposes. The operation process is described in detail, and the successful operation mainly depends on effective reagents, special Escherichia coli strains, and basic molecular biological means without other specific equipments.

Indexed as

Transformation, GeneticAgrobacteriumCrops, AgriculturalGenetic VectorsMetabolic EngineeringPlants, Genetically ModifiedPlasmidsRecombinasesRecombination, GeneticSynthetic BiologyTransgenesRecombinasesCre/loxPGene assemblyMetabolic engineeringMultigene stackingSynthetic biology

Identifiers

PMID33471322

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.