Evidence map›Paper›PMID 33469579›Full record

ArticlebioRxiv : the preprint server for biology2021

Novel RT-ddPCR Assays for determining the transcriptional profile of SARS-CoV-2.

Sushama Telwatte, Nitasha Kumar, Albert Vallejo-Gracia, G Renuka Kumar, Chuanyi M Lu, Melanie Ott, Joseph K Wong, Steven A Yukl

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Sushama TelwatteDepartment of Medicine, University of California, San Francisco, CA, USA.
Nitasha KumarDepartment of Medicine, University of California, San Francisco, CA, USA.
Albert Vallejo-GraciaGladstone Institute of Virology, Gladstone Institutes, San Francisco, CA, USA.
G Renuka KumarGladstone Institute of Virology, Gladstone Institutes, San Francisco, CA, USA.
Chuanyi M LuDepartment of Medicine, University of California, San Francisco, CA, USA.
Melanie OttDepartment of Medicine, University of California, San Francisco, CA, USA.
Joseph K WongDepartment of Medicine, University of California, San Francisco, CA, USA.
Steven A YuklDepartment of Medicine, University of California, San Francisco, CA, USA.

Funding

Virology CoreP30AI027763 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI STEVEN Grant DEEKS · 1988 to 2026
$93.6M
Novel single genome approaches to determine the mechanisms of HIV latent infection in blood, gut, and lymph nodesR01DK120387 · NIDDK · NORTHERN CALIFORNIA INSTITUTE/RES/EDU · PI LICHTERFELD, MATHIAS, YUKL, STEVEN A · 2019 to 2023
$4.1M
Investigating Mechanisms of HIV Persistence in the GutR01DK108349 · NIDDK · NORTHERN CALIFORNIA INSTITUTE/RES/EDU · PI YUKL, STEVEN A · 2015 to 2019
$2.2M
Understanding HIV latency reversal and clearance of infected cells in vivoR01AI132128 · NIAID · NORTHERN CALIFORNIA INSTITUTE/RES/EDU · PI WONG, JOSEPH K, YUKL, STEVEN A · 2017 to 2021
$1.9M
NIAID NIH HHS P30 AI027763NIAID NIH HHS R01 AI132128NIDDK NIH HHS R01 DK108349NIDDK NIH HHS R01 DK120387
6 · The paper itself

Abstract

The exact mechanism of coronavirus replication and transcription is not fully understood; however, a hallmark of coronavirus transcription is the generation of negative-sense RNA intermediates that serve as the templates for the synthesis of positive-sense genomic RNA (gRNA) and an array of subgenomic mRNAs (sgRNAs) encompassing sequences arising from discontinuous transcription. Existing PCR-based diagnostic assays for SAR-CoV-2 are qualitative or semi-quantitative and do not provide the resolution needed to assess the complex transcription dynamics of SARS-CoV-2 over the course of infection. We developed and validated a novel panel of specially designed SARS-CoV-2 ddPCR-based assays to map the viral transcription profile. Application of these assays to clinically relevant samples will enhance our understanding of SARS-CoV-2 replication and transcription and may also inform the development of improved diagnostic tools and therapeutics.

Indexed as

coronavirusdroplet-digital PCRquantitative assaysSARS-CoV-2viral transcription/replication

Identifiers

PMID33469579
PMCPMC7814816

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.