Evidence map›Paper›PMID 33469577›Full record

ArticlebioRxiv : the preprint server for biology2021

Tropism of SARS-CoV-2 for Developing Human Cortical Astrocytes.

Madeline G Andrews, Tanzila Mukhtar, Ugomma C Eze, Camille R Simoneau, Yonatan Perez, Mohammed A Mostajo-Radji, Shaohui Wang, Dmitry Velmeshev, Jahan Salma, G Renuka Kumar and 4 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 51 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 2 countries.

Madeline G AndrewsDepartment of Neurology, University of California, San Francisco (UCSF), San Francisco, CA, USA.
Tanzila MukhtarDepartment of Neurology, University of California, San Francisco (UCSF), San Francisco, CA, USA.
Ugomma C EzeDepartment of Neurology, University of California, San Francisco (UCSF), San Francisco, CA, USA.
Camille R SimoneauGladstone Institutes, San Francisco, CA, USA.
Yonatan PerezDepartment of Neurology, University of California, San Francisco (UCSF), San Francisco, CA, USA.
Mohammed A Mostajo-RadjiDepartment of Neurology, University of California, San Francisco (UCSF), San Francisco, CA, USA.
Shaohui WangDepartment of Neurology, University of California, San Francisco (UCSF), San Francisco, CA, USA.
Dmitry VelmeshevDepartment of Neurology, University of California, San Francisco (UCSF), San Francisco, CA, USA.
Jahan SalmaCenter for Regenerative Medicine and Stem Cell Research, The Aga Khan University, Karachi, Pakistan.
G Renuka KumarGladstone Institutes, San Francisco, CA, USA.
Alex A PollenDepartment of Neurology, University of California, San Francisco (UCSF), San Francisco, CA, USA.
Elizabeth E CrouchThe Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, UCSF, San Francisco, CA, USA.
Melanie OttGladstone Institutes, San Francisco, CA, USA.
Arnold R KriegsteinDepartment of Neurology, University of California, San Francisco (UCSF), San Francisco, CA, USA.
University of California, San Francisco · USGladstone Institutes · USAga Khan University · PK

Funding

MEDICAL SCIENTIST TRAINING PROGRAMT32GM007618 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ANDERSON, MARK S · 1985 to 2020
$29.6M
Development and Expansion of the Human Cerebral CortexR35NS097305 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI KRIEGSTEIN, ARNOLD · 2017 to 2024
$8.5M
A Cellular Resolution Census of the Developing Human BrainU01MH114825 · NIMH · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI HUANG, ERIC J, KRIEGSTEIN, ARNOLD · 2017 to 2021
$6.7M
A new model of accelerated immune aging in HIV-infected drug usersDP1DA038043 · NIDA · J. DAVID GLADSTONE INSTITUTES · PI OTT, MELANIE MARIA · 2014 to 2019
$4.8M
Improving Brain Organoid Models by Mediating Metabolic DysregulationK99MH125329 · NIMH · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ANDREWS, MADELINE · 2020 to 2021
$164k
NIDA NIH HHS DP1 DA038043NIGMS NIH HHS T32 GM007618NIMH NIH HHS K99 MH125329NIMH NIH HHS U01 MH114825NINDS NIH HHS R35 NS097305
6 · The paper itself

Abstract

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) readily infects a variety of cell types impacting the function of vital organ systems, with particularly severe impact on respiratory function. It proves fatal for one percent of those infected. Neurological symptoms, which range in severity, accompany a significant proportion of COVID-19 cases, indicating a potential vulnerability of neural cell types. To assess whether human cortical cells can be directly infected by SARS-CoV-2, we utilized primary human cortical tissue and stem cell-derived cortical organoids. We find significant and predominant infection in cortical astrocytes in both primary and organoid cultures, with minimal infection of other cortical populations. Infected astrocytes had a corresponding increase in reactivity characteristics, growth factor signaling, and cellular stress. Although human cortical cells, including astrocytes, have minimal ACE2 expression, we find high levels of alternative coronavirus receptors in infected astrocytes, including DPP4 and CD147. Inhibition of DPP4 reduced infection and decreased expression of the cell stress marker, ARCN1. We find tropism of SARS-CoV-2 for human astrocytes mediated by DPP4, resulting in reactive gliosis-type injury.

Identifiers

PMID33469577
PMCPMC7814814
OpenAlexW3124615634

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.