ArticleFrontiers in molecular biosciences2020
Protein and Organic-Molecular Crystallography With 300kV Electrons on a Direct Electron Detector.
Article in Frontiers in molecular biosciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Self-stretching molecular monolayers with autonomous folding and mechanical adaptation.Science advances · 2026Article
- Analytical Ultracentrifugation in Aotearoa: A Brief History of the Technique and its Place Among Other Tools Used to Investigate Biomolecular Interactions.Journal of the Royal Society of New Zealand · 2026Review
- Two-dimensional metal-organic frameworks offer all-in-one cocatalysts for photocatalytic overall water splitting.Nature chemistry · 2026Article
- Expanded segments of three-dimensional carbonaceous nets with chirality: synthesis and structures.Chemical science · 2025Article
- Standards for MicroED.Acta crystallographica. Section C, Structural chemistry · 2025Article
- Fast event-based electron counting for small-molecule structure determination by MicroED.Acta crystallographica. Section C, Structural chemistry · 2025Article
- 3D Electron Diffraction Structure of an Organic Semiconductor Reveals Conformational Polymorphism.Journal of the American Chemical Society · 2025Article
- Advances and applications of microcrystal electron diffraction (MicroED).Current opinion in structural biology · 2024Review
- Exploration and development of molecule-based printed electronics materials: an integrated approach using experimental, computational, and data sciences.Science and technology of advanced materials · 2024Review
- Enantioselectivity of discretized helical supramolecule consisting of achiral cobalt phthalocyanines via chiral-induced spin selectivity effect.Nature communications · 2023Article
- Machine learning for classifying narrow-beam electron diffraction data.Acta crystallographica. Section A, Foundations and advances · 2023Article
- Structural resolution of a small organic molecule by serial X-ray free-electron laser and electron crystallography.Nature chemistry · 2023Article
- Electron-counting MicroED data with the K2 and K3 direct electron detectors.Journal of structural biology · 2022Article
- Electron Diffraction of 3D Molecular Crystals.Chemical reviews · 2022Review
- Ab initio phasing macromolecular structures using electron-counted MicroED data.Nature methods · 2022Article
- Electron crystallography of chiral and non-chiral small molecules.Ultramicroscopy · 2022Article
- 3D Electron Diffraction for Chemical Analysis: Instrumentation Developments and Innovative Applications.Chemical reviews · 2021Review
- Machine learning-based real-time object locator/evaluator for cryo-EM data collection.Communications biology · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electron 3D crystallography can reveal the atomic structure from undersized crystals of various samples owing to the strong scattering power of electrons. Here, a direct electron detector DE64 was tested for small and thin crystals of protein and an organic molecule using a JEOL CRYO ARM 300 electron microscope. The microscope is equipped with a cold-field emission gun operated at an accelerating voltage of 300 kV, quad condenser lenses for parallel illumination, an in-column energy filter, and a stable rotational goniometer stage. Rotational diffraction data were collected in an unsupervised manner from crystals of a heme-binding enzyme catalase and a representative organic semiconductor material Ph-BTBT-C10. The structures were determined by molecular replacement for catalase and by the direct method for Ph-BTBT-C10. The analyses demonstrate that the system works well for electron 3D crystallography of these molecules with less damaging, a smaller point spread, and less noise than using the conventional scintillator-coupled camera.
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