ArticleScientific reports2021
Genetic manipulation of pathogenic Leptospira: CRISPR interference (CRISPRi)-mediated gene silencing and rapid mutant recovery at 37 °C.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 37 citations in OpenAlex.
- Identification and characterization of small RNAs expressed by Leptospira borgpetersenii serovar Hardjo and their conservation in the genus Leptospira.BMC microbiology · 2026Article
- TheInfection and immunity · 2026Article
- CRISPR-based mutagenesis of lipopolysaccharide biosynthesis genes in Leptospira interrogans reveals gene essentiality and confirms the role of an O-antigen polymerase.Scientific reports · 2026Article
- Leptospira and Leptospirosis: A Review of Species Classifications, Genomes, Morphological Structures, Antimicrobial Resistances, Transmissions, and Clinical Manifestations.Current microbiology · 2026Review
- Creating a Library of Random Transposon Mutants in Leptospira.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Gene Knockout in Leptospira spp. by CRISPR/Cas9-NHEJ (Non-Homologous End-Joining) and CRISPR-Prime Editing.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Transposon Sequencing in Leptospira interrogans.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Bioinformatic Identification of CRISPR-Cas Systems inPathogens (Basel, Switzerland) · 2025Article
- Article
- Induced protein expression in Leptospira spp. and its application to CRISPR/Cas9 mutant generation.Scientific reports · 2025Article
- Progress and persistence of diseases of high consequence to livestock in the United States.One health (Amsterdam, Netherlands) · 2024Review
- Article
- Leptospira interrogans encodes a canonical BamA and three novel noNterm Omp85 outer membrane protein paralogs.Scientific reports · 2024Article
- Leptospiral adhesins: from identification to future perspectives.Frontiers in microbiology · 2024Review
- Concurrent colonization of rodent kidneys with multiple species and serogroups of pathogenicApplied and environmental microbiology · 2023Article
- LipL41 and LigA/LigB Gene Silencing on a LipL32 KnockoutPathogens (Basel, Switzerland) · 2023Article
- The Arsenal ofAntioxidants (Basel, Switzerland) · 2023Review
- Evaluation ofFrontiers in microbiology · 2023Article
- Putative Pathogenic Genes ofTropical medicine and infectious disease · 2022Article
- Diverse lineages of pathogenic Leptospira species are widespread in the environment in Puerto Rico, USA.PLoS neglected tropical diseases · 2022Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leptospirosis is a neglected, widespread zoonosis caused by pathogenic species of the genus Leptospira, and is responsible for 60,000 deaths per year. Pathogenic mechanisms of leptospirosis remain poorly understood mainly because targeted mutations or gene silencing in pathogenic Leptospira continues to be inherently inefficient, laborious, costly and difficult to implement. In addition, pathogenic leptospires are highly fastidious and the selection of mutants on solid agar media can take up to 6 weeks. The catalytically inactive Cas9 (dCas9) is an RNA-guided DNA-binding protein from the Streptococcus pyogenes CRISPR/Cas system and can be used for gene silencing, in a strategy termed CRISPR interference (CRISPRi). Here, this technique was employed to silence genes encoding major outer membrane proteins of pathogenic L. interrogans. Conjugation protocols were optimized using the newly described HAN media modified for rapid mutant recovery at 37 °C in 3% CO
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