ArticleScientific reports2021
A novel and sensitive real-time PCR system for universal detection of poxviruses.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- Fish Poxviruses on the Rise: Prospects for Aquatic Health.Journal of fish diseases · 2026Review
- Advanced strategies for the diagnosis, treatment, and vaccination of monkeypox.Journal of nanobiotechnology · 2026Review
- Real-Time and Nanoplate-Based Digital PCR Assays for the Detection and Absolute Quantification of Borealpox Virus.International journal of molecular sciences · 2026Article
- Modelling the effectiveness of an isolation strategy for managing mpox outbreaks with variable infectiousness profiles.Nature communications · 2024Article
- A Comparative Evaluation of Three Diagnostic Assays for the Detection of Human Monkeypox.Viruses · 2024Article
- Metagenomic and Molecular Detection of Novel Fecal Viruses in Free-Ranging Agile Wallabies.EcoHealth · 2023Article
- Mapping the Landscape of Health Research Priorities for Effective Pandemic Preparedness in Human Mpox Virus Disease.Pathogens (Basel, Switzerland) · 2023Review
- Current Status of Epidemiology, Diagnosis, Therapeutics, and Vaccines for the Re-Emerging Human Monkeypox Virus.Journal of microbiology and biotechnology · 2023Review
- Molecular detection of monkeypox and related viruses: challenges and opportunities.Virus genes · 2023Review
- Internal Validation of a Real-Time qPCR Kit following the UNE/EN ISO/IEC 17025:2005 for Detection of the Re-EmergingDiagnostics (Basel, Switzerland) · 2023Article
- Rapid Reverse Purification DNA Extraction Approaches to Identify Microbial Pathogens in Wastewater.Microorganisms · 2023Article
- Monkeypox: another pandemic in the making?Proceedings (Baylor University. Medical Center) · 2023Review
- Monkeypox Virus Infections in Humans.Clinical microbiology reviews · 2022Review
- Current and Perspective Sensing Methods for Monkeypox Virus.Bioengineering (Basel, Switzerland) · 2022Review
- Article
- Article
- Monkeypox: another test for PCR.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2022Article
Corrections and comments
- Erratum issued
Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Success in smallpox eradication was enabled by the absence of non-human reservoir for smallpox virus. However, other poxviruses with a wider host spectrum can infect humans and represent a potential health threat to humans, highlighted by a progressively increasing number of infections by (re)emerging poxviruses, requiring new improved diagnostic and epidemiological tools. We describe here a real-time PCR assay targeting a highly conserved region of the poxvirus genome, thus allowing a pan-Poxvirus detection (Chordopoxvirinae and Entomopoxvirinae). This system is specific (99.8% for vertebrate samples and 99.7% for arthropods samples), sensitive (100% for vertebrate samples and 86.3% for arthropods samples) and presents low limit of detection (< 1000 DNA copies/reaction). In addition, this system could be also valuable for virus discovery and epidemiological projects.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.