Evidence map›Paper›PMID 33468229›Full record

ArticleBMC research notes2021

Genetic diversity of Giardia isolates from patients in Chandigarh region: India.

Shabnam Thakur, Upninder Kaur, Rakesh Sehgal

Abstract read
In one paragraph

Article in BMC research notes, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Molecular appraisal ofTropical parasitology
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Shabnam ThakurDepartment of Medical Parasitology, Postgraduate Institute of Medical Research and Education, Chandigarh, 160012, India.
Upninder KaurDepartment of Medical Parasitology, Postgraduate Institute of Medical Research and Education, Chandigarh, 160012, India.
Rakesh SehgalDepartment of Medical Parasitology, Postgraduate Institute of Medical Research and Education, Chandigarh, 160012, India. sehgalpgi@gmail.com.ORCID http://orcid.org/0000-0003-2910-4973

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThe aim of study was to characterize Giardia isolates genetically among patients in Chandigarh region, India. For this, nested PCR targeting fragment of the glutamate dehydrogenase (GLUD1 earlier named as GDH) gene was used. Phylogenetic analysis was done by constructing neighbor-joining tree made out of the nucleotide sequences of G. intestinalis isolates obtained in this study and with the known sequences published in GenBank.

resultsOut of 40 samples, GLUD1 gene was amplified in 33 samples (82.5%). The product of GLUD1 gene was successfully sequenced only in 32 samples. In these samples, assemblage B was found in 27 (84.37%) samples whereas 5 (15.6%) samples had assemblage A. Among assemblage B most of them were of BIII. Therefore, genotyping of Giardia would be helpful in conducting epidemiological studies.

Indexed as

Giardia lambliaGiardiasisFecesGenetic VariationGenotypeGiardiaHumansIndiaPhylogenyGenotypeGiardiaGLUD1 (glutamate dehydrogenase)PCRSequencing

Identifiers

PMID33468229
PMCPMC7816347

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