ArticleJournal of nanobiotechnology2021
Few-layer graphene induces both primary and secondary genotoxicity in epithelial barrier models in vitro.
Article in Journal of nanobiotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- Preliminary Study to Investigate Possible Cyto-Genotoxic and Oxidative Effects of Few-Layer Graphene in Human Bronchial Cells.International journal of molecular sciences · 2024Article
- Adapting the in vitro micronucleus assay (OECD Test Guideline No. 487) for testing of manufactured nanomaterials: recommendations for best practices.Mutagenesis · 2024Article
- Environmental and Health Impacts of Graphene and Other Two-Dimensional Materials: A Graphene Flagship Perspective.ACS nano · 2024Review
- Review
- Current status and future challenges of genotoxicity OECD Test Guidelines for nanomaterials: a workshop report.Mutagenesis · 2023Article
- The State of the Art and Challenges of In Vitro Methods for Human Hazard Assessment of Nanomaterials in the Context of Safe-by-Design.Nanomaterials (Basel, Switzerland) · 2023Review
- Role of Nanomaterials in the Fabrication of bioNEMS/MEMS for Biomedical Applications and towards Pioneering Food Waste Utilisation.Nanomaterials (Basel, Switzerland) · 2022Review
- Lung recovery from DNA damage induced by graphene oxide is dependent on size, dose and inflammation profile.Particle and fibre toxicology · 2022Article
- Genotoxicity of Graphene-Based Materials.Nanomaterials (Basel, Switzerland) · 2022Review
- The application of existing genotoxicity methodologies for grouping of nanomaterials: towards an integrated approach to testing and assessment.Particle and fibre toxicology · 2022Article
- Primary and Secondary Genotoxicity of Nanoparticles: Establishing a Co-Culture Protocol for Assessing Micronucleus Using Flow Cytometry.Frontiers in toxicology · 2022Article
- Direct and Indirect Genotoxicity of Graphene Family Nanomaterials on DNA-A Review.Nanomaterials (Basel, Switzerland) · 2021Review
- In Vitro Assessment of the Genotoxic Potential of Pristine Graphene Platelets.Nanomaterials (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundToxicological evaluation of engineered nanomaterials (ENMs) is essential for occupational health and safety, particularly where bulk manufactured ENMs such as few-layer graphene (FLG) are concerned. Additionally, there is a necessity to develop advanced in vitro models when testing ENMs to provide a physiologically relevant alternative to invasive animal experimentation. The aim of this study was to determine the genotoxicity of non-functionalised (neutral), amine- and carboxyl-functionalised FLG upon both human-transformed type-I (TT1) alveolar epithelial cell monocultures, as well as co-cultures of TT1 and differentiated THP-1 monocytes (d.THP-1 (macrophages)).
resultsIn monocultures, TT1 and d.THP-1 macrophages showed a statistically significant (p < 0.05) cytotoxic response with each ENM following 24-h exposures. Monoculture genotoxicity measured by the in vitro cytokinesis blocked micronucleus (CBMN) assay revealed significant (p < 0.05) micronuclei induction at 8 µg/ml for amine- and carboxyl-FLG. Transmission electron microscopy (TEM) revealed ENMs were internalised by TT1 cells within membrane-bound vesicles. In the co-cultures, ENMs induced genotoxicity in the absence of cytotoxic effects. Co-cultures pre-exposed to 1.5 mM N-acetylcysteine (NAC), showed baseline levels of micronuclei induction, indicating that the genotoxicity observed was driven by oxidative stress.
conclusionsTherefore, FLG genotoxicity when examined in monocultures, results in primary-indirect DNA damage; whereas co-cultured cells reveal secondary mechanisms of DNA damage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.