Evidence map›Paper›PMID 33467918›Full record

ArticleBioTechniques2021

Urea-based amino sugar agent clears murine liver and preserves protein fluorescence and lipophilic dyes.

Michelle Hough, Michael Fenlon, Alison Glazier, Celia Short, Gerardo Esteban Fernandez, Jiabo Xu, Elaa Mahdi, Kinji Asahina, Kasper S Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioTechniques, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 58% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Michelle HoughDevelopmental Biology, Regenerative Medicine & Stem Cell Program, The Saban Research Institute, Children's Hospital of Los Angeles, Los Angeles, CA 90027, USA.
Michael FenlonDevelopmental Biology, Regenerative Medicine & Stem Cell Program, The Saban Research Institute, Children's Hospital of Los Angeles, Los Angeles, CA 90027, USA.
Alison GlazierDevelopmental Biology, Regenerative Medicine & Stem Cell Program, The Saban Research Institute, Children's Hospital of Los Angeles, Los Angeles, CA 90027, USA.
Celia ShortDevelopmental Biology, Regenerative Medicine & Stem Cell Program, The Saban Research Institute, Children's Hospital of Los Angeles, Los Angeles, CA 90027, USA.
Gerardo Esteban FernandezDevelopmental Biology, Regenerative Medicine & Stem Cell Program, The Saban Research Institute, Children's Hospital of Los Angeles, Los Angeles, CA 90027, USA.
Jiabo XuDevelopmental Biology, Regenerative Medicine & Stem Cell Program, The Saban Research Institute, Children's Hospital of Los Angeles, Los Angeles, CA 90027, USA.
Elaa MahdiDevelopmental Biology, Regenerative Medicine & Stem Cell Program, The Saban Research Institute, Children's Hospital of Los Angeles, Los Angeles, CA 90027, USA.
Kinji AsahinaSouthern California Research Center for ALPD & Cirrhosis, Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90089, USA.
Kasper S WangDevelopmental Biology, Regenerative Medicine & Stem Cell Program, The Saban Research Institute, Children's Hospital of Los Angeles, Los Angeles, CA 90027, USA.ORCID 0000-0003-0487-6416
Children's Hospital of Los Angeles · USUniversity of Southern California · US

Funding

Limited Competition for the Continuation of the Childhood Liver Disease Research Network (ChilLDRen) Clinical Centers.U01DK084538 · NIDDK · CHILDREN'S HOSPITAL OF LOS ANGELES · PI KOHLI, ROHIT · 2009 to 2023
$4.8M
Dissection of Glisson's capsule in alcohol induced liver injuryR21AA024840 · NIAAA · UNIVERSITY OF SOUTHERN CALIFORNIA · PI ASAHINA, KINJI · 2016 to 2017
$433k
NIAAA NIH HHS R21 AA024840NIDDK NIH HHS U01 DK084538
6 · The paper itself

Abstract

Five established clearing protocols were compared with a modified and simplified method to determine an optimal clearing reagent for three-dimensionally visualizing fluorophores in the murine liver, a challenging organ to clear. We report successful clearing of whole liver lobes by modification of an established protocol (UbasM) using only Ub-1, a urea-based amino sugar reagent, in a simpler protocol that requires only a 24-h processing time. With Ub-1 alone, we observed sufficiently preserved liver tissue structure in three dimensions along with excellent preservation of fluorophore emissions from endogenous protein reporters and lipophilic tracer dyes. This streamlined technique can be used for 3D cell lineage tracing and fluoroprobe-based reporter gene expression to compare various experimental conditions.

Indexed as

Amino SugarsFluorescent DyesUreaAnimalsFluorescenceLiverMiceAmino SugarsFluorescent DyesUrea3D imagingbile duct ligationcomputational imagingfluorescence microscopylipophilic tracersliver clearingoptical clearing agentsprominin-1

Identifiers

PMID33467918
PMCPMC7983039
OpenAlexW3124938993

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.