ArticleMetabolic engineering2021
Transportome-wide engineering of Saccharomyces cerevisiae.
Article in Metabolic engineering, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 50 citations in OpenAlex.
- Synthesis of Amines for Active Pharmaceutical Ingredients Using the Whole-Cell Factory Saccharomyces Cerevisae.Chembiochem : a European journal of chemical biology · 2026Review
- Unveiling Specificity, Redundancy, and Promiscuity of FiveInternational journal of molecular sciences · 2026Article
- Engineering Saccharomyces cerevisiae to improve heterologous abscisic acid production.Microbial cell factories · 2026Article
- Whole-cell and cell-free biosensor-driven metabolic engineering.Current opinion in biotechnology · 2025Review
- Genetically encoded biosensor enabled mining, characterisation and engineering of aromatic acid MFS transporters.Journal of biological engineering · 2025Article
- High-throughput metabolic engineering ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- A previously unidentified sugar transporter for engineering of high-yield Streptomyces.Applied microbiology and biotechnology · 2024Article
- Transportation engineering for enhanced production of plant natural products in microbial cell factories.Synthetic and systems biotechnology · 2024Review
- Evidence for the Role of the Mitochondrial ABC Transporter MDL1 in the Uptake of Clozapine and Related Molecules into the YeastPharmaceuticals (Basel, Switzerland) · 2024Article
- Identification of transporters involved in aromatic compounds tolerance through screening of transporter deletion libraries.Microbial biotechnology · 2024Article
- Coupling High-Throughput and Targeted Screening for Identification of Nonobvious Metabolic Engineering Targets.ACS synthetic biology · 2024Article
- Mevalonate secretion is not mediated by a singular non-essential transporter inBiotechnology notes (Amsterdam, Netherlands) · 2024Article
- Deorphanizing solute carriers inFrontiers in microbiology · 2024Article
- Identification and expression of small multidrug resistance transporters in early-branching anaerobic fungi.Protein science : a publication of the Protein Society · 2023Article
- TransAAP: an automated annotation pipeline for membrane transporter prediction in bacterial genomes.Microbial genomics · 2023Article
- Screening ofFrontiers in microbiology · 2023Article
- High throughput mutagenesis and screening for yeast engineering.Journal of biological engineering · 2022Review
- Review
- A Chemogenomic Toolkit to Evaluate the "Ins and Outs" of Yeast Plasma Membrane Transporters.mBio · 2022Article
- Biosensor-CoupledmSystems · 2022Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synthetic biology enables the production of small molecules by recombinant microbes for pharma, food, and materials applications. The secretion of products reduces the cost of separation and purification, but it is challenging to engineer due to the limited understanding of the transporter proteins' functions. Here we describe a method for genome-wide transporter disruption that, in combination with a metabolite biosensor, enables the identification of transporters impacting the production of a given target metabolite in yeast Saccharomyces cerevisiae. We applied the method to study the transport of xenobiotic compounds, cis,cis-muconic acid (CCM), protocatechuic acid (PCA), and betaxanthins. We found 22 transporters that influenced the production of CCM or PCA. The transporter of the 12-spanner drug:H(+) antiporter (DHA1) family Tpo2p was further confirmed to import CCM and PCA in Xenopus expression assays. We also identified three transporter proteins (Qdr1p, Qdr2p, and Apl1p) involved in betaxanthins transport. In summary, the described method enables high-throughput transporter identification for small molecules in cell factories.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.