Evidence map›Paper›PMID 33465374›Full record

ArticleThe Journal of biological chemistry

Mapping membrane lipids in the developing and adult mouse retina under physiological and pathological conditions using mass spectrometry.

Fumie Hamano, Hiroshi Kuribayashi, Toshiro Iwagawa, Asano Tsuhako, Katsuyuki Nagata, Hiroshi Sagara, Takao Shimizu, Hideo Shindou, Sumiko Watanabe

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.1field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 15 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Single-Cell Transcriptomic Profiling of Müller Glia in the rd10 Retina.Advances in experimental medicine and biology · 2023
    Article
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Fumie HamanoDepartment of Lipid Signaling, National Center for Global Health and Medicine, Shinjuku-ku, Tokyo, Japan; Life Sciences Core Facility, Graduate School of Medicine, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Hiroshi KuribayashiDivision of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan.
Toshiro IwagawaDivision of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan.
Asano TsuhakoDivision of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan.
Katsuyuki NagataDepartment of Lipid Signaling, National Center for Global Health and Medicine, Shinjuku-ku, Tokyo, Japan.
Hiroshi SagaraMedical Proteomics Laboratory, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan.
Takao ShimizuDepartment of Lipid Signaling, National Center for Global Health and Medicine, Shinjuku-ku, Tokyo, Japan.
Hideo ShindouDepartment of Lipid Signaling, National Center for Global Health and Medicine, Shinjuku-ku, Tokyo, Japan; Department of Lipid Science, Graduate School of Medicine, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Sumiko WatanabeDivision of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan. Electronic address: sumiko@ims.u-tokyo.ac.jp.
National Center for Global Health and Medicine · JPThe University of Tokyo · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Membrane phospholipids play pivotal roles in various cellular processes, and their levels are tightly regulated. In the retina, phospholipids had been scrutinized because of their distinct composition and requirement in visual transduction. However, how lipid composition changes during retinal development remains unclear. Here, we used liquid chromatography-mass spectrometry (LC-MS) to assess the dynamic changes in the levels of two main glycerophospholipids, phosphatidylcholine (PC) and phosphatidylethanolamine (PE), in the developing mouse retina under physiological and pathological conditions. The total levels of PC and PE increased during retinal development, and individual lipid species exhibited distinct level changes. The amount of very-long-chain PC and PE increased dramatically in the late stages of retinal development. The mRNA levels of Elovl2 and Elovl4, genes encoding enzymes essential for the synthesis of very-long-chain polyunsaturated fatty acids, increased in developing photoreceptors. Cell sorting based on CD73 expression followed by LC-MS revealed distinct changes in PC and PE levels in CD73-positive rod photoreceptors and CD73-negative retinal cells. Finally, using the NaIO

Indexed as

Gene Expression Regulation, Developmental5'-NucleotidaseAcyltransferasesAnimalsChromatography, LiquidEye ProteinsFatty Acid DesaturasesFatty Acid ElongasesFatty Acids, UnsaturatedFemaleFlow CytometryIodatesMass SpectrometryMembrane LipidsMembrane ProteinsMice2-acylglycerophosphate acyltransferase5'-NucleotidaseAcyltransferasesELOVL2 protein, mouseElovl4 protein, mouseEye ProteinsFADS2 protein, mouseFatty Acid DesaturasesFatty Acid ElongasesFatty Acids, UnsaturatedIodatesMembrane LipidsMembrane ProteinsPhosphatidylcholinesphosphatidylethanolaminePhosphatidylethanolaminesRNA, Messengersodium iodate

Identifiers

PMID33465374
PMCPMC7949107
OpenAlexW3125197372

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.