Evidence map›Paper›PMID 33463919›Full record

ArticleCritical reviews in eukaryotic gene expression2020

Bioactivity-Guided Isolation and Identification of Anti-adipogenic Constituents from the n-Butanol Fraction of Cissus quadrangularis.

Rabail Hassan Toor, Zainab Nasir Khan, Maira Tariq, Raazia Tassaduq, Qurratulann Afza Gardner, W Waheed-Uz-Zaman, Jane B Lian, Janet L Stein, Gary S Stein, Abdul Rauf Shakoori

Abstract read
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In one paragraph

Article in Critical reviews in eukaryotic gene expression, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Effects ofFood science of animal resources · 2024
    Article
  2. Lupenone-Rich Fraction Derived fromLife (Basel, Switzerland) · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Rabail Hassan ToorSchool of Biological Sciences, University of the Punjab, Quaid-i-Azam Campus, Lahore, Pakistan.
Zainab Nasir KhanSchool of Biological Sciences, University of the Punjab, Quaid-i-Azam Campus, Lahore, Pakistan.
Maira TariqSchool of Biological Sciences, University of the Punjab, Quaid-i-Azam Campus, Lahore, Pakistan.
Raazia TassaduqDepartment of Biochemistry, Kinnaird College for Women, Lahore, Pakistan.
Qurratulann Afza GardnerSchool of Biological Sciences, University of the Punjab, Quaid-i-Azam Campus, Lahore, Pakistan.
W Waheed-Uz-ZamanInstitute of Chemistry, University of the Punjab, Quaid-i-Azam Campus, Lahore, Pakistan.
Jane B LianDepartment of Biochemistry, Robert Larner College of Medicine, University of Vermont, Burlington, Vermont, USA.
Janet L SteinDepartment of Biochemistry, Robert Larner College of Medicine, University of Vermont, Burlington, Vermont, USA.
Gary S SteinDepartment of Biochemistry, Robert Larner College of Medicine, University of Vermont, Burlington, Vermont, USA.
Abdul Rauf ShakooriSchool of Biological Sciences, University of the Punjab, Quaid-i-Azam Campus, Lahore, Pakistan.

Funding

CONTROL OF OSTEOBLAST PROLIFERATION AND DIFFERENTIATIONR01AR039588 · NIAMS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI LIAN, JANE B., STEIN, GARY S. · 1990 to 2019
$8.0M
Chromatin Organization Regulates OsteogenesisR01DE029311 · NIDCR · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI LIAN, JANE B., STEIN, GARY S. · 2020 to 2024
$2.5M
NIAMS NIH HHS R01 AR039588NIDCR NIH HHS R01 DE029311
6 · The paper itself

Abstract

Obesity is marked by the buildup of fat in adipose tissue that increases body weight and the risk of many associated health problems, including diabetes and cardiovascular disease. Treatment options for obesity are limited, and available medications have many side effects. Thus there is a great need to find alternative medicines for treating obesity. This study explores the anti-adipogenic potential of the n-butanol fraction of Cissus quadrangularis (CQ-B) on 3T3-L1 mouse preadipocyte cell line. The expression of various lipogenic marker genes such as adiponectin, peroxisome proliferator-activated receptor gamma, leptin, fatty acid-binding proteins, sterol regulatory element-binding proteins, fetal alcohol syndrome, steroyl-CoA desaturase-1, lipoproteins, acetyl-CoA carboxylase alpha, and acetyl-CoA carboxylase beta were variously significantly downregulated. After establishing the anti-adipogenic potential of CQ-B, it was fractionated to isolate anti-adipogenic compounds. We observed significant reduction in neutral lipid content of differentiated cells treated with various fractions of CQ-B. Gas chromatography-mass spectrometry analysis revealed the presence of thirteen compounds with reported anti-adipogenic activities. Further studies to purify these compounds can offer efficacious and viable treatment options for obesity and related complications.

Indexed as

3T3-L1 CellsAcetyl-CoA CarboxylaseAdipogenesisAdiponectinAnimalsCissusFatty Acid-Binding ProteinsFatty Acid DesaturasesGene Expression RegulationHumansLeptinMiceObesityPlant ExtractsPPAR gammaSterol Regulatory Element Binding Protein 1ACACA protein, humanAcetyl-CoA CarboxylaseAdiponectinFatty Acid-Binding ProteinsFatty Acid DesaturasesLeptinPlant ExtractsPPAR gammaSterol Regulatory Element Binding Protein 1

Identifiers

PMID33463919
PMCPMC8650164

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.