ArticleACS biomaterials science & engineering2020
Engineering Aligned Skeletal Muscle Tissue Using Decellularized Plant-Derived Scaffolds.
Article in ACS biomaterials science & engineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 118 citations in OpenAlex.
- Hierarchical Silica-Cellulose Nanoarchitectures from Rice Straw: A Waste-to-Value Platform for Autonomous Osteogenic Bone Regeneration.Journal of functional biomaterials · 2026Article
- Growing Relevance of Decellularized Plant Material as Functional Scaffolds in Tissue Engineering and Cultured Meat: A Scoping Review on Recent Advances, Challenges, and Future Directions.Annals of biomedical engineering · 2026Review
- Engineering a Compartmentalized Multi-Cell Co-Culture Hydrogel System Using Beeswax/Fucoidan/Alginate for Cultured Meat Modeling.Foods (Basel, Switzerland) · 2026Article
- Emergent directional persistence in fibrous granular scaffolds guides myotube organization.bioRxiv : the preprint server for biology · 2026Article
- Plant-Based Scaffolds in Tissue Engineering: Structure-Function, Processing, and Clinical Outlook-A Review.Annals of biomedical engineering · 2026Review
- Fabrication and Characterization of Electrospun PCL/GelMA Composite Scaffolds for Muscle Tissue Engineering.Tissue engineering and regenerative medicine · 2025Article
- Chick embryo extract-driven culture system enhances chicken muscle satellite cell differentiation and 3D myotube formation.NPJ science of food · 2025Article
- Cellulose Plant-Derived Scaffolds as a Tool for Myometrium Modeling.International journal of molecular sciences · 2025Article
- Engineering vascular grafts from decellularized plants: Advances and challenges.Histology and histopathology · 2025Review
- Plant-Based Scaffolds for Tissue Engineering: A Review.Polymers · 2025Review
- A Tissue Engineering's Guide to Biomimicry.Macromolecular bioscience · 2025Review
- Hyaluronic Acid-Coated Melt Electrowritten Scaffolds Promote Myoblast Attachment, Alignment, and Differentiation.Cellular and molecular bioengineering · 2025Article
- Hyaluronic Acid-Coated Melt Electrowritten Scaffolds Promote Myoblast Attachment, Alignment, and Differentiation.bioRxiv : the preprint server for biology · 2025Article
- Bioprinting Vascularized Constructs for Clinical Relevance: Engineering Hydrogel Systems for Biological Maturity.Gels (Basel, Switzerland) · 2025Review
- Decellularized apple hypanthium as a plant-based biomaterial for cartilage regeneration in vitro: a comparative study of progenitor cell types and environmental conditions.Journal of biological engineering · 2025Article
- Optimization and Standardization of Plant-Derived Vascular Scaffolds.International journal of molecular sciences · 2025Article
- Cultivation of bovine lipid chunks on Aloe vera scaffolds.NPJ science of food · 2025Article
- Growing meat on autoclaved vegetables with biomimetic stiffness and micro-patterns.Nature communications · 2025Article
- The potential function of soy protein hydrolysate to induce myogenic differentiation of C2C12 cells.PloS one · 2025Article
- Biocompatibility of decellularized spinach leaves.npj biomedical innovations · 2025Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
To achieve organization and function, engineered tissues require a scaffold that supports cell adhesion, alignment, growth, and differentiation. For skeletal muscle tissue engineering, decellularization has been an approach for fabricating 3D scaffolds that retain biological architecture. While many decellularization approaches are focused on utilizing animal muscle as the starting material, decellularized plants are a potential source of highly structured cellulose-rich scaffolds. Here, we assessed the potential for a variety of decellularized plant scaffolds to promote mouse and human muscle cell alignment and differentiation. After decellularizing a range of fruits and vegetables, we identified the green-onion scaffold to have appropriate surface topography for generating highly confluent and aligned C2C12 and human skeletal muscle cells (HSMCs). The topography of the green-onion cellulose scaffold contained a repeating pattern of grooves that are approximately 20 μm wide by 10 μm deep. The outer white section of the green onion had a microstructure that guided C2C12 cell differentiation into aligned myotubes. Quantitative analysis of C2C12 and HSMC alignment revealed an almost complete anisotropic organization compared to 2D isotropic controls. Our results demonstrate that the decellularized green onion cellulose scaffolds, particularly from the outer white bulb segment, provide a simple and low-cost substrate to engineer aligned human skeletal muscle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.