ArticleScientific reports2021
Endogenous zebrafish proneural Cre drivers generated by CRISPR/Cas9 short homology directed targeted integration.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- In vivo single-cell RNA metabolic labeling resolves early transcriptional responders in the regenerating zebrafish heart.Nature communications · 2026Article
- Advancing knock-in approaches for robust genome editing in zebrafish.Biology open · 2026Article
- Lineage labeling with zebrafish hand2 Cre and CreERT2 recombinase CRISPR knock-ins.Developmental dynamics : an official publication of the American Association of Anatomists · 2026Article
- Advancing Knock-In Approaches for Robust Genome Editing in Zebrafish.bioRxiv : the preprint server for biology · 2025Article
- Advances in CRISPR-Cas9 in lineage tracing of model animals.Animal models and experimental medicine · 2025Review
- Zebrafish as a Versatile Model for Cardiovascular Research: Peering into the Heart of the Matter.Cells · 2025Review
- Fluorescent Tagging of Endogenous FOXO for Live Imaging and Pull-Down Assays.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Lineage labeling with zebrafishbioRxiv : the preprint server for biology · 2024Article
- Article
- The role of cilia during organogenesis in zebrafish.Open biology · 2023Review
- Efficient knock-in method enabling lineage tracing in zebrafish.Life science alliance · 2023Article
- Genome editing.Scientific reports · 2022Article
- Rbbp4 loss disrupts neural progenitor cell cycle regulation independent of Rb and leads to Tp53 acetylation and apoptosis.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- Cre/eLife · 2022Article
- GeneWeld: Efficient Targeted Integration Directed by Short Homology in Zebrafish.Bio-protocol · 2021Article
- How Zebrafish Can Drive the Future of Genetic-based Hearing and Balance Research.Journal of the Association for Research in Otolaryngology : JARO · 2021Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
We previously reported efficient precision targeted integration of reporter DNA in zebrafish and human cells using CRISPR/Cas9 and short regions of homology. Here, we apply this strategy to isolate zebrafish Cre recombinase drivers whose spatial and temporal restricted expression mimics endogenous genes. A 2A-Cre recombinase transgene with 48 bp homology arms was targeted into proneural genes ascl1b, olig2 and neurod1. We observed high rates of germline transmission ranging from 10 to 100% (2/20 olig2; 1/5 neurod1; 3/3 ascl1b). The transgenic lines Tg(ascl1b-2A-Cre)
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.