ArticleScientific reports2021
The effect of taxonomic classification by full-length 16S rRNA sequencing with a synthetic long-read technology.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 84 papers, 3 of them syntheses that pooled it.
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Who cites it
84 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- Unboxing the black box-one step forward to understand the soil microbiome: A systematic review.Microbial ecology · 2023Pooled it
- Meta-analysis of caries microbiome studies can improve upon disease prediction outcomes.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2022Pooled it
- Predicting cancer immunotherapy response from gut microbiomes using machine learning models.Oncotarget · 2022Pooled it
- Five-Day Treatment withNutrients · 2025Trial
- Correlation Between Microbial Communities and Volatile Organic Compounds in Camel Milk at Different Lactation Stages in Xinjiang, China.Foods (Basel, Switzerland) · 2026Article
- Translating Gut Microbiota into Diagnostics: A Multidimensional Approach for the Diagnosis of Inflammatory Bowel Disease.Gut and liver · 2026Review
- Species-level profiling ofPeerJ · 2026Article
- Mapping the Vaginal Metabolic Profile in Dysbiosis, Persistent Human Papillomavirus Infection, and Cervical Intraepithelial Neoplasia: A Scoping Review.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Key Challenges in Plant Microbiome Research in the Next Decade.Microorganisms · 2025Review
- Fungus-infected Meiogyne flowers offer a brood site for beetle pollinators in a tripartite nursery pollination system.Annals of botany · 2025Article
- Ecological management of the microbiota in patients with cancer.Nature reviews. Clinical oncology · 2025Review
- Toward standardized methods in canine vaginal microbiome research: evaluation of storage, host DNA depletion, and database selection.Microbiology spectrum · 2025Article
- Consistent microbial insights across sequencing methods in soil studies: the role of reference taxonomies.mSystems · 2025Article
- Morpho-biochemical and molecular characterization of Bacillus subtilis and Priestia megaterium isolates from eastern Indian farmlands.Molecular biology reports · 2025Article
- Call for caution on alleged tick-borne pathogens in blood-fed ticks from animals across nine Asian countries.Microbiology spectrum · 2025Article
- Backtracking identification techniques for predicting unclear bacterial taxonomy at species level: molecular diagnosis-based bacterial classification.Genes & genomics · 2025Article
- Differences in cell-associated and cell-free microbial DNA in blood.bioRxiv : the preprint server for biology · 2025Article
- Multidisciplinary methodologies used in the study of cable bacteria.FEMS microbiology reviews · 2025Review
- Effects of dietary supplementation of nitrate on enteric methane production, performance, and rumen microbiome of Hanwoo steers.Journal of animal science · 2025Article
- Laboratory and In-Field Metagenomics for Environmental Monitoring.Methods in molecular biology (Clifton, N.J.) · 2025Article
24 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Characterizing the microbial communities inhabiting specimens is one of the primary objectives of microbiome studies. A short-read sequencing platform for reading partial regions of the 16S rRNA gene is most commonly used by reducing the cost burden of next-generation sequencing (NGS), but misclassification at the species level due to its length being too short to consider sequence similarity remains a challenge. Loop Genomics recently proposed a new 16S full-length-based synthetic long-read sequencing technology (sFL16S). We compared a 16S full-length-based synthetic long-read (sFL16S) and V3-V4 short-read (V3V4) methods using 24 human GUT microbiota samples. Our comparison analyses of sFL16S and V3V4 sequencing data showed that they were highly similar at all classification resolutions except the species level. At the species level, we confirmed that sFL16S showed better resolutions than V3V4 in analyses of alpha-diversity, relative abundance frequency and identification accuracy. Furthermore, we demonstrated that sFL16S could overcome the microbial misidentification caused by different sequence similarity in each 16S variable region through comparison the identification accuracy of Bifidobacterium, Bacteroides, and Alistipes strains classified from both methods. Therefore, this study suggests that the new sFL16S method is a suitable tool to overcome the weakness of the V3V4 method.
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