ArticleScientific reports2021
An optimised method for intact nuclei isolation from diatoms.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Article
- Comprehensive review on the potential of ultrasound for blue food protein extraction, modification and impact on bioactive properties.Ultrasonics sonochemistry · 2024Review
- The Diatom Diversity and Ecological Status of a Tufa-Depositing River through eDNA Metabarcoding vs. a Morphological Approach-A Case Study of the Una River (Bosnia and Herzegovina).Microorganisms · 2024Article
- Review
- Gene expression during the formation of resting spores induced by nitrogen starvation in the marine diatom Chaetoceros socialis.BMC genomics · 2023Article
- Gene expression variations and allele-specific expression of two rice and their hybrid in caryopses at single-nucleus resolution.Frontiers in plant science · 2023Article
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Authors and funding
8 authors.
Funding
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Abstract
Due to their abundance in the oceans, their extraordinary biodiversity and the increasing use for biotech applications, the study of diatom biology is receiving more and more attention in the recent years. One of the limitations in developing molecular tools for diatoms lies in the peculiar nature of their cell wall, that is made of silica and organic molecules and that hinders the application of standard methods for cell lysis required, for example, to extract organelles. In this study we present a protocol for intact nuclei isolation from diatoms that was successfully applied to three different species: two pennates, Pseudo-nitzschia multistriata and Phaeodactylum tricornutum, and one centric diatom species, Chaetoceros diadema. Intact nuclei were extracted by treatment with acidified NH
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