ArticleeLife2021
Freshwater monitoring by nanopore sequencing.
Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
53 citing papers in PubMed.
- Sub-Nanomolar Detection and Discrimination of Microcystin Congeners Using Aerolysin Nanopores.ACS nano · 2026Article
- Conditional superiorities and unaddressed bottlenecks: a critical review of artificial intelligence for waterborne microbial detection.Applied microbiology and biotechnology · 2026Review
- Long-read nanopore shotgun metagenomic DNA sequencing for river biodiversity, wildlife, pollution, and environmental health monitoring.NAR genomics and bioinformatics · 2026Article
- MMonitor for real-time monitoring of microbial communities using long reads.Cell reports methods · 2026Article
- Cell-free DNA in sepsis: from molecular insights to clinical management.Military Medical Research · 2025Review
- Going Mobile: Using Portable Genomic Technologies for PCR-Free In Situ Species Identification and Real-Time Molecular Systematics.Ecology and evolution · 2025Article
- Article
- Nanopore Environmental Analysis.JACS Au · 2025Review
- Cas9-based enrichment for targeted long-read metabarcoding.Royal Society open science · 2025Article
- Microbial Ecotoxicology-40 Years on.Life (Basel, Switzerland) · 2025Article
- Nanopore- and AI-empowered microbial viability inference.GigaScience · 2025Article
- Evaluation of nanopore sequencing for increasing accessibility of eDNA studies in biodiverse countries.PloS one · 2025Article
- Influence of sewage effluent discharge on putative pathogen community in drinking water sources: insights from full-length 16S rRNA gene amplicon sequencing.Journal of water and health · 2025Article
- Custom barcoded primers for influenza A nanopore sequencing: enhanced performance with reduced preparation time.Frontiers in cellular and infection microbiology · 2025Article
- Emerging technologies and current challenges in intratumoral microbiota research.Frontiers in cellular and infection microbiology · 2025Review
- Using short-read 16S rRNA sequencing of multiple variable regions to generate high-quality results to a species level.Frontiers in bioinformatics · 2025Article
- Article
- Sterile sentinels and MinION sequencing capture active soil microbial communities that differentiate crop rotations.Environmental microbiome · 2024Article
- Citizen science is a vital partnership for invasive alien species management and research.iScience · 2024Review
- Air monitoring by nanopore sequencing.ISME communications · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
Abstract
While traditional microbiological freshwater tests focus on the detection of specific bacterial indicator species, including pathogens, direct tracing of all aquatic DNA through metagenomics poses a profound alternative. Yet, in situ metagenomic water surveys face substantial challenges in cost and logistics. Here, we present a simple, fast, cost-effective and remotely accessible freshwater diagnostics workflow centred around the portable nanopore sequencing technology. Using defined compositions and spatiotemporal microbiota from surface water of an example river in Cambridge (UK), we provide optimised experimental and bioinformatics guidelines, including a benchmark with twelve taxonomic classification tools for nanopore sequences. We find that nanopore metagenomics can depict the hydrological core microbiome and fine temporal gradients in line with complementary physicochemical measurements. In a public health context, these data feature relevant sewage signals and pathogen maps at species level resolution. We anticipate that this framework will gather momentum for new environmental monitoring initiatives using portable devices.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.