ArticleVirology journal2021
Autophagy is involved in the replication of H9N2 influenza virus via the regulation of oxidative stress in alveolar epithelial cells.
Article in Virology journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 36 citations in OpenAlex.
- Fangchinoline restores TFEB-driven lysosomal biogenesis and blocks H1N1 infection.Acta pharmacologica Sinica · 2026Article
- Research Progress on Avian Influenza Virus and Autophagy: A Review.Pathogens (Basel, Switzerland) · 2026Review
- A novel mechanistic study on inhibiting influenza A virus replication by a newly extracted polypeptide targeting host autophagy.Archives of microbiology · 2025Article
- EpithelialMedComm · 2025Article
- Autophagy and Respiratory Viruses: Mechanisms, Viral Exploitation, and Therapeutic Insights.Cells · 2025Review
- A double-edged sword in antiviral defence: ATG7 binding dicer to promote virus replication.Cellular and molecular life sciences : CMLS · 2025Article
- Article
- The Roles of Endocytosis and Autophagy at the Cellular Level During Influenza Virus Infection: A Mini-Review.Infection and drug resistance · 2024Review
- Effect of natural products on host cell autophagy induced by Influenza A virus infection.Frontiers in cellular and infection microbiology · 2024Review
- Understanding host response to infectious salmon anaemia virus in an Atlantic salmon cell line using single-cell RNA sequencing.BMC genomics · 2023Article
- Protective effects of pentoxifylline against chlorine-induced acute lung injury in rats.BMC pharmacology & toxicology · 2023Article
- The battle for autophagy between host and influenza A virus.Virulence · 2022Review
- The Multi-Faceted Role of Autophagy During Animal Virus Infection.Frontiers in cellular and infection microbiology · 2022Review
- Coxsackievirus B3 infection induces glycolysis to facilitate viral replication.Frontiers in microbiology · 2022Article
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOxidative stress is an important pathogenic factor in influenza A virus infection. It has been found that reactive oxygen species induced by the H9N2 influenza virus is associated with viral replication. However, the mechanisms involved remain to be elucidated.
methodsIn this study, the role of autophagy was investigated in H9N2 influenza virus-induced oxidative stress and viral replication in A549 cells. Autophagy induced by H9N2 was inhibited by an autophagy inhibitor or RNA interference, the autophagy level, viral replication and the presence of oxidative stress were detected by western blot, TCID50 assay, and Real-time PCR. Then autophagy and oxidative stress were regulated, and viral replication was determined. At last, the Akt/TSC2/mTOR signaling pathways was detected by western blot.
resultsAutophagy was induced by the H9N2 influenza virus and the inhibition of autophagy reduced the viral titer and the expression of nucleoprotein and matrix protein. The blockage of autophagy suppressed the H9N2 virus-induced increase in the presence of oxidative stress, as evidenced by decreased reactive oxygen species production and malonaldehyde generation, and increased superoxide dismutase 1 levels. The changes in the viral titer and NP mRNA level caused by the antioxidant, N-acetyl-cysteine (NAC), and the oxidizing agent, H
conclusionAutophagy is involved in H9N2 virus replication by regulating oxidative stress via the Akt/TSC2/mTOR signaling pathway. Thus, autophagy maybe a target which may be used to improve antiviral therapeutics.
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