Evidence map›Paper›PMID 33460645›Full record

ReviewExperimental neurology2021

Serotonergic innervation of respiratory motor nuclei after cervical spinal injury: Impact of intermittent hypoxia.

Marissa C Ciesla, Yasin B Seven, Latoya L Allen, Kristin N Smith, Zachary A Asa, Alec K Simon, Ashley E Holland, Juliet V Santiago, Kelsey Stefan, Ashley Ross and 2 more

Open access · greenAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Experimental neurology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 22 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Marissa C CieslaBreathing Research and Therapeutics Center, Department of Physical Therapy & McKnight Brain Institute, University of Florida, FL 32610, USA.
Yasin B SevenBreathing Research and Therapeutics Center, Department of Physical Therapy & McKnight Brain Institute, University of Florida, FL 32610, USA.
Latoya L AllenBreathing Research and Therapeutics Center, Department of Physical Therapy & McKnight Brain Institute, University of Florida, FL 32610, USA.
Kristin N SmithBreathing Research and Therapeutics Center, Department of Physical Therapy & McKnight Brain Institute, University of Florida, FL 32610, USA.
Zachary A AsaBreathing Research and Therapeutics Center, Department of Physical Therapy & McKnight Brain Institute, University of Florida, FL 32610, USA.
Alec K SimonBreathing Research and Therapeutics Center, Department of Physical Therapy & McKnight Brain Institute, University of Florida, FL 32610, USA.
Ashley E HollandBreathing Research and Therapeutics Center, Department of Physical Therapy & McKnight Brain Institute, University of Florida, FL 32610, USA.
Juliet V SantiagoBreathing Research and Therapeutics Center, Department of Physical Therapy & McKnight Brain Institute, University of Florida, FL 32610, USA.
Kelsey StefanBreathing Research and Therapeutics Center, Department of Physical Therapy & McKnight Brain Institute, University of Florida, FL 32610, USA.
Ashley RossBreathing Research and Therapeutics Center, Department of Physical Therapy & McKnight Brain Institute, University of Florida, FL 32610, USA.
Elisa J Gonzalez-RothiBreathing Research and Therapeutics Center, Department of Physical Therapy & McKnight Brain Institute, University of Florida, FL 32610, USA.
Gordon S MitchellBreathing Research and Therapeutics Center, Department of Physical Therapy & McKnight Brain Institute, University of Florida, FL 32610, USA. Electronic address: gsmitche@phhp.ufl.edu.
Allen Institute for Brain Science · USUniversity of Florida · US

Funding

Rehabilitation Research Career Development ProgramK12HD055929 · NICHD · UNIVERSITY OF TEXAS MED BR GALVESTON · PI BROWN, DAVID A · 2007 to 2021
$11.7M
Functional mapping of peripheral and central circuits for airway protection and breathingOT2OD023854 · OD · UNIVERSITY OF FLORIDA · PI BOLSER, DONALD C · 2016 to 2020
$10.8M
Training In Rehabilitation and Neuromuscular PlasticityT32HD043730 · NICHD · UNIVERSITY OF FLORIDA · PI DAVID D FULLER · 2003 to 2026
$5.3M
Respiratory plasticity and spinal cord InjuryR37HL069064 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI MITCHELL, GORDON S. · 2002 to 2011
$4.2M
Optimizing respiratory plasticity with chronic cervical SCIR01HL147554 · NHLBI · UNIVERSITY OF FLORIDA · PI MITCHELL, GORDON S. · 2019 to 2022
$2.9M
Respiratory Plasticity and Spinal Cord InjuryR01HL069064 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI MITCHELL, GORDON S. · 2014 to 2017
$1.5M
NHLBI NIH HHS R01 HL069064NHLBI NIH HHS R01 HL147554NHLBI NIH HHS R37 HL069064NICHD NIH HHS K12 HD055929NICHD NIH HHS T32 HD043730NIH HHS OT2 OD023854
6 · The paper itself

Abstract

Although cervical spinal cord injury (cSCI) disrupts bulbo-spinal serotonergic projections, partial recovery of spinal serotonergic innervation below the injury site is observed after incomplete cSCI. Since serotonin contributes to functional recovery post-injury, treatments to restore or accelerate serotonergic reinnervation are of considerable interest. Intermittent hypoxia (IH) was reported to increase serotonin innervation near respiratory motor neurons in spinal intact rats, and to improve function after cSCI. Here, we tested the hypotheses that spontaneous serotonergic reinnervation of key respiratory (phrenic and intercostal) motor nuclei: 1) is partially restored 12 weeks post C2 hemisection (C2Hx); 2) is enhanced by IH; and 3) results from sprouting of spared crossed-spinal serotonergic projections below the site of injury. Serotonin was assessed via immunofluorescence in male Sprague Dawley rats with and without C2Hx (12 wks post-injury); individual groups were exposed to 28 days of: 1) normoxia; 2) daily acute IH (dAIH28: 10, 5 min 10.5% O2 episodes per day; 5 min normoxic intervals); 3) mild chronic IH (IH28-5/5: 5 min 10.5% O2 episodes; 5 min intervals; 8 h/day); or 4) moderate chronic IH (IH28-2/2: 2 min 10.5% O2 episodes; 2 min intervals; 8 h/day), simulating IH experienced during moderate sleep apnea. After C2Hx, the number of ipsilateral serotonergic structures was decreased in both motor nuclei, regardless of IH protocol. However, serotonergic structures were larger after C2Hx in both motor nuclei, and total serotonin immunolabeling area was increased in the phrenic motor nucleus but reduced in the intercostal motor nucleus. Both chronic IH protocols increased serotonin structure size and total area in the phrenic motor nuclei of uninjured rats, but had no detectable effects after C2Hx. Although the functional implications of fewer but larger serotonergic structures are unclear, we confirm that serotonergic reinnervation is substantial following injury, but IH does not affect the extent of reinnervation.

Indexed as

HypoxiaAnimalsCervical CordCervical VertebraeIntercostal NervesMaleMotor NeuronsNerve RegenerationPhrenic NerveRatsRats, Sprague-DawleyRecovery of FunctionSerotoninSpinal Cord InjuriesSerotoninCervical hemisectionIntercostal motor neuronsIntermittent hypoxiaPhrenic motor neuronsRapheSerotoninSpinal cord injury

Identifiers

PMID33460645
PMCPMC8327480
OpenAlexW3118281286

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.