ArticleBiophysical journal2021
Influence of membrane-cortex linkers on the extrusion of membrane tubes.
Article in Biophysical journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- A tutorial for mesoscale computer simulations of lipid membranes: tether pulling, tubulation and fluctuations.Soft matter · 2025Review
- Glycocalyx-induced formation of membrane tubes.Biophysical journal · 2025Article
- Review
- Making the cut: Multiscale simulation of membrane remodeling.Current opinion in structural biology · 2024Review
- Cell Membrane Tension Gradients, Membrane Flows, and Cellular Processes.Physiology (Bethesda, Md.) · 2024Review
- Viscoelastic phenotyping of red blood cells.Biophysical journal · 2024Article
- Tension-induced adhesion mode switching: the interplay between focal adhesions and clathrin-containing adhesion complexes.bioRxiv : the preprint server for biology · 2024Article
- Article
- Probing mechanical interaction of immune receptors and cytoskeleton by membrane nanotube extraction.Scientific reports · 2023Article
- Mesoscale simulations: An indispensable approach to understand biomembranes.Biophysical journal · 2023Review
- Modeling membrane reshaping driven by dynamic protein assemblies.Current opinion in structural biology · 2023Review
- Effective cell membrane tension is independent of polyacrylamide substrate stiffness.PNAS nexus · 2023Article
- Actomyosin-Driven Division of a Synthetic Cell.ACS synthetic biology · 2022Review
- Cytoplasmic forces functionally reorganize nuclear condensates in oocytes.Nature communications · 2022Article
- Rapid propagation of membrane tension at retinal bipolar neuron presynaptic terminals.Science advances · 2022Article
- Physical mechanisms of ESCRT-III-driven cell division.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Review
- Biomechanics of Neutrophil Tethers.Life (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cell membrane is an inhomogeneous system composed of phospholipids, sterols, carbohydrates, and proteins that can be directly attached to underlying cytoskeleton. The protein linkers between the membrane and the cytoskeleton are believed to have a profound effect on the mechanical properties of the cell membrane and its ability to reshape. Here, we investigate the role of membrane-cortex linkers on the extrusion of membrane tubes using computer simulations and experiments. In simulations, we find that the force for tube extrusion has a nonlinear dependence on the density of membrane-cortex attachments: at a range of low and intermediate linker densities, the force is not significantly influenced by the presence of the membrane-cortex attachments and resembles that of the bare membrane. For large concentrations of linkers, however, the force substantially increases compared with the bare membrane. In both cases, the linkers provided membrane tubes with increased stability against coalescence. We then pulled tubes from HEK cells using optical tweezers for varying expression levels of the membrane-cortex attachment protein Ezrin. In line with simulations, we observed that overexpression of Ezrin led to an increased extrusion force, while Ezrin depletion had a negligible effect on the force. Our results shed light on the importance of local protein rearrangements for membrane reshaping at nanoscopic scales.
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